CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance
Fil: Abrate, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/acceptedVersion |
| Language: | eng |
| Published: |
2025
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| Online Access: | http://hdl.handle.net/11086/556882 https://doi.org/10.1080/2162402X.2025.2502354 |
| _version_ | 1839930204870410240 |
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| author | Abrate, Carolina Canale, Fernando P. Bossio, Sabrina N. Tosello Boari, Jimena Ramello, María C. Nuñez, Nicolas Richer, Wilfrid Sedlik, Christine Denizeau, Jordan Vincent-Salomon, Anne Borcoman, Edith Del Castillo, Andres Gruppi, Adriana Acosta Rodríguez, Eva V. Piaggio, Eliane Montes, Carolina L. |
| author2 | https://orcid.org/0000-0001-5805-6785 |
| author_facet | https://orcid.org/0000-0001-5805-6785 Abrate, Carolina Canale, Fernando P. Bossio, Sabrina N. Tosello Boari, Jimena Ramello, María C. Nuñez, Nicolas Richer, Wilfrid Sedlik, Christine Denizeau, Jordan Vincent-Salomon, Anne Borcoman, Edith Del Castillo, Andres Gruppi, Adriana Acosta Rodríguez, Eva V. Piaggio, Eliane Montes, Carolina L. |
| author_sort | Abrate, Carolina |
| collection | Repositorio Digital Universitario |
| description | Fil: Abrate, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. |
| format | info:eu-repo/semantics/acceptedVersion |
| id | rdu-unc.556882 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2025 |
| record_format | dspace |
| spelling | rdu-unc.5568822025-08-07T15:17:02Z CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance Abrate, Carolina Canale, Fernando P. Bossio, Sabrina N. Tosello Boari, Jimena Ramello, María C. Nuñez, Nicolas Richer, Wilfrid Sedlik, Christine Denizeau, Jordan Vincent-Salomon, Anne Borcoman, Edith Del Castillo, Andres Gruppi, Adriana Acosta Rodríguez, Eva V. Piaggio, Eliane Montes, Carolina L. https://orcid.org/0000-0001-5805-6785 https://orcid.org/0000-0002-6789-6118 https://orcid.org/0000-0003-1563-4420 https://orcid.org/0000-0001-8108-141X https://orcid.org/0000-0003-3837-270X https://orcid.org/0000-0003-1802-3529 https://orcid.org/0000-0001-8502-0905 https://orcid.org/0000-0001-5754-5771 https://orcid.org/0000-0002-5393-5657 https://orcid.org/0000-0002-8034-9303 https://orcid.org/0000-0001-5871-2391 https://orcid.org/0000-0002-2455-8442 https://orcid.org/0000-0003-0494-1820 Breast cancer CD8+ T cells Exhaustion PD-1 Draining lymph nodes info:eu-repo/semantics/acceptedVersion Fil: Abrate, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Abrate, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Canale, Fernando P. University of Zürich. Institute of Experimental Immunology; Switzerland. Fil: Bossio, Sabrina N. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Bossio, Sabrina N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Tosello Boari, Jimena. PSL Research University. Institut Curie Research Center; France. Fil: Ramello, María C. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Ramello, María C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Nuñez, Nicolas. PSL Research University. Institut Curie Research Center; France. Fil: Richer, Wilfrid. PSL Research University. Institut Curie Research Center; France. Fil: Sedlik, Christine. PSL Research University. Institut Curie Research Center; France. Fil: Denizeau, Jordan. PSL Research University. Institut Curie Research Center; France. Fil: Vincent-Salomon, Anne. PSL Research University. Institut Curie; France. Fil: Borcoman, Edith. PSL Research University. Institut Curie Research Center; France. Fil: Borcoman, Edith. Institut Curie. Department of Medical Oncology; France. Fil: Del Castillo, Andres. Hospital Rawson. Departamento de Mastología y Ginecología; Argentina. Fil: Gruppi, Adriana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Gruppi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Acosta Rodríguez, Eva V. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Acosta Rodríguez, Eva V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Piaggio, Eliane. PSL Research University. Institut Curie Research Center; France. Fil: Montes, Carolina L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Montes, Carolina L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. CD8+ T cells shape the antitumor immune response. Here, we evaluated CD8+ T cells expressing different levels of PD-1, their functional status, and distribution in different tissues of luminal breast cancer (BC) patients. We characterized the exhaustion stages of CD8+ T cells in tumors, juxtatumoral tissues (JTs), and tumor-draining lymph nodes (TDLNs). Terminal exhausted CD8+ T cells (PD-1<sup>High</sup> CD8+) were predominant in tumors and nearly absent in other tissues. However, in all tissues evaluated, most CD8+ T cells exhibited a pre-exhausted phenotype (PD-1<sup>Int</sup> CD8+) or did not express PD-1. PD-1<sup>High</sup> and PD-1<sup>Int</sup> CD8+ T cells from tumors and JTs presented central and effector memory phenotypes, while in TDLNs were primarily central memory. TCR-β sequencing revealed higher clonality among CD8+ T cells from tumor than TDLNs, with tumor-enriched clones also detected in TDLNs. Analysis of scRNA-seq datasets from tumors and JTs of colorectal and non-small cell lung cancer patients, identified a CD8+ terminal exhaustion and a CD8+ pre-exhausted signatures. High expression of exhaustion-associated genes in BC tumors correlated with improved overall survival. Overall, PD-1 expression effectively distinguishes exhaustion stages in CD8+ T cells. PD-1<sup>Int</sup> cells found in tumors, JTs, and TDLNs represent a promising therapeutic target for cancer immunotherapy. info:eu-repo/semantics/acceptedVersion Fil: Abrate, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Abrate, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Canale, Fernando P. University of Zürich. Institute of Experimental Immunology; Switzerland. Fil: Bossio, Sabrina N. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Bossio, Sabrina N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Tosello Boari, Jimena. PSL Research University. Institut Curie Research Center; France. Fil: Ramello, María C. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Ramello, María C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Nuñez, Nicolas. PSL Research University. Institut Curie Research Center; France. Fil: Richer, Wilfrid. PSL Research University. Institut Curie Research Center; France. Fil: Sedlik, Christine. PSL Research University. Institut Curie Research Center; France. Fil: Denizeau, Jordan. PSL Research University. Institut Curie Research Center; France. Fil: Vincent-Salomon, Anne. PSL Research University. Institut Curie; France. Fil: Borcoman, Edith. PSL Research University. Institut Curie Research Center; France. Fil: Borcoman, Edith. Institut Curie. Department of Medical Oncology; France. Fil: Del Castillo, Andres. Hospital Rawson. Departamento de Mastología y Ginecología; Argentina. Fil: Gruppi, Adriana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Gruppi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Acosta Rodríguez, Eva V. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Acosta Rodríguez, Eva V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Piaggio, Eliane. PSL Research University. Institut Curie Research Center; France. Fil: Montes, Carolina L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Montes, Carolina L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. 2025-08-07T14:03:59Z 2025-08-07T14:03:59Z 2025 article Abrate, C., Canale, F. P., Bossio, S. N., Tosello Boari, J., Ramello, M. C., Nuñez, N., … Montes, C. L. (2025). CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effector functions and prognostic relevance. OncoImmunology, 14(1). https://doi.org/10.1080/2162402X.2025.2502354 Copy citation to clipboard http://hdl.handle.net/11086/556882 2162-4011 https://doi.org/10.1080/2162402X.2025.2502354 eng Licencia Creative Commons Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Breast cancer CD8+ T cells Exhaustion PD-1 Draining lymph nodes Abrate, Carolina Canale, Fernando P. Bossio, Sabrina N. Tosello Boari, Jimena Ramello, María C. Nuñez, Nicolas Richer, Wilfrid Sedlik, Christine Denizeau, Jordan Vincent-Salomon, Anne Borcoman, Edith Del Castillo, Andres Gruppi, Adriana Acosta Rodríguez, Eva V. Piaggio, Eliane Montes, Carolina L. CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title | CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title_full | CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title_fullStr | CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title_full_unstemmed | CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title_short | CD8+ T cells in breast cancer tumors and draining lymph nodes: PD-1 levels, effectorfunctions and prognostic relevance |
| title_sort | cd8 t cells in breast cancer tumors and draining lymph nodes pd 1 levels effectorfunctions and prognostic relevance |
| topic | Breast cancer CD8+ T cells Exhaustion PD-1 Draining lymph nodes |
| url | http://hdl.handle.net/11086/556882 https://doi.org/10.1080/2162402X.2025.2502354 |
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