Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD)
Objective: Detecting traces of ancient DNA of Vibrio cholerae to provide genetic information associated with the fifth cholera pandemic.Materials: Sediment samples from the sacral foramina of four individuals were analyzed, recovered from a mass grave near an institution dedicated exclusively to the...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Elsevier
2021
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| Online Access: | http://hdl.handle.net/11336/142747 https://suquia.ffyh.unc.edu.ar/handle/suquia/175322 |
| _version_ | 1826515252758446080 |
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| author | Ramirez, Darío Alejandro Saka, Hector Alex Nores, Rodrigo |
| author_facet | Ramirez, Darío Alejandro Saka, Hector Alex Nores, Rodrigo |
| author_sort | Ramirez, Darío Alejandro |
| collection | Repositorio Suquia |
| description | Objective: Detecting traces of ancient DNA of Vibrio cholerae to provide genetic information associated with the fifth cholera pandemic.Materials: Sediment samples from the sacral foramina of four individuals were analyzed, recovered from a mass grave near an institution dedicated exclusively to the isolation and treatment of citizens infected with cholera in the late 19th century in the city of Cordoba, Argentina.Methods: Paleogenetic techniques (ancient DNA extraction, PCR amplification, and Sanger sequencing) were applied. Specific primers for Vibrio cholerae (VCR, ctxA, ctxB, and tcpA) were designed.Results: By amplifying and sequencing the Vibrio cholerae repeats fragment, the infection in at least one individual was confirmed.Conclusions: The synthesis of the paleogenetic results with the archaeological and historical evidence strongly supports that at least one individual from the mass grave in Cordoba, Argentina, was a victim of the fifth cholera pandemic.Significance: Confirming the presence of the disease through multiple lines of evidence, including genetic, archaeological, and historical analyses, strengthens and affirms our understanding of the presence, effects, and potential evolutionary paths of the disease in the past.Limitations: Vibrio cholerae repeats were sequenced in one individual, while the remaining genes could not be amplified, which is likely related to gene copy number.Suggestions for future research: Paleogenetic examination of ancient samples from different locations will broaden our understanding of the origin, evolution, and past dissemination of Vibrio cholerae epidemic strains. |
| format | info:eu-repo/semantics/article |
| id | suquia-ffyh.175322 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2021 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | suquia-ffyh.1753222024-08-02T01:23:14Z Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) Ramirez, Darío Alejandro Saka, Hector Alex Nores, Rodrigo PALEOGENETICS PALEOMICROBIOLOGY SEDIMENTS ANCIENT BACTERIA INFECTIOUS DISEASES CHOLERA COVID-19 https://purl.org/becyt/ford/6.5 https://purl.org/becyt/ford/6 Objective: Detecting traces of ancient DNA of Vibrio cholerae to provide genetic information associated with the fifth cholera pandemic.Materials: Sediment samples from the sacral foramina of four individuals were analyzed, recovered from a mass grave near an institution dedicated exclusively to the isolation and treatment of citizens infected with cholera in the late 19th century in the city of Cordoba, Argentina.Methods: Paleogenetic techniques (ancient DNA extraction, PCR amplification, and Sanger sequencing) were applied. Specific primers for Vibrio cholerae (VCR, ctxA, ctxB, and tcpA) were designed.Results: By amplifying and sequencing the Vibrio cholerae repeats fragment, the infection in at least one individual was confirmed.Conclusions: The synthesis of the paleogenetic results with the archaeological and historical evidence strongly supports that at least one individual from the mass grave in Cordoba, Argentina, was a victim of the fifth cholera pandemic.Significance: Confirming the presence of the disease through multiple lines of evidence, including genetic, archaeological, and historical analyses, strengthens and affirms our understanding of the presence, effects, and potential evolutionary paths of the disease in the past.Limitations: Vibrio cholerae repeats were sequenced in one individual, while the remaining genes could not be amplified, which is likely related to gene copy number.Suggestions for future research: Paleogenetic examination of ancient samples from different locations will broaden our understanding of the origin, evolution, and past dissemination of Vibrio cholerae epidemic strains. Fil: Ramirez, Darío Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Antropología de Córdoba. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Instituto de Antropología de Córdoba; Argentina Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Nores, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Antropología de Córdoba. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Instituto de Antropología de Córdoba; Argentina 2021-03 2024-08-02T01:23:14Z 2024-08-02T01:23:14Z info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/11336/142747 Ramirez, Darío Alejandro; Saka, Hector Alex; Nores, Rodrigo; Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD); Elsevier; International Journal of Paleopathology; 32; 3-2021; 74-79 1879-9817 CONICET Digital CONICET https://suquia.ffyh.unc.edu.ar/handle/suquia/175322 eng info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1879981720300711 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpp.2020.12.004 info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf application/pdf application/pdf Elsevier |
| spellingShingle | PALEOGENETICS PALEOMICROBIOLOGY SEDIMENTS ANCIENT BACTERIA INFECTIOUS DISEASES CHOLERA COVID-19 https://purl.org/becyt/ford/6.5 https://purl.org/becyt/ford/6 Ramirez, Darío Alejandro Saka, Hector Alex Nores, Rodrigo Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title | Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title_full | Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title_fullStr | Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title_full_unstemmed | Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title_short | Detection of Vibrio cholerae aDNA in human burials from the fifth cholera pandemic in Argentina (1886–1887 AD) |
| title_sort | detection of vibrio cholerae adna in human burials from the fifth cholera pandemic in argentina 1886 1887 ad |
| topic | PALEOGENETICS PALEOMICROBIOLOGY SEDIMENTS ANCIENT BACTERIA INFECTIOUS DISEASES CHOLERA COVID-19 https://purl.org/becyt/ford/6.5 https://purl.org/becyt/ford/6 |
| url | http://hdl.handle.net/11336/142747 https://suquia.ffyh.unc.edu.ar/handle/suquia/175322 |
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