Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloac...
| Main Authors: | , , |
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| Format: | Online |
| Language: | spa |
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Facultad de Ciencias Agropecuarias
1992
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| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/2369 |
| _version_ | 1834605838405206016 |
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| author | Altube, H. A. Cabello, F. Ortiz, J. M. |
| author_facet | Altube, H. A. Cabello, F. Ortiz, J. M. |
| author_sort | Altube, H. A. |
| collection | Portal de Revistas |
| description | Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), malate dehydrogenases (MDH), and peroxidases (PER) were separated using polyacrylamide gels and later stained with specific stains for the various isoenzyme systems. ACPH, CO, EST, and MDH were found to be the most adequate systems for characterization. The results of the study of the different systems and the grouping of the cultivars are presented. The use of isoenzymatic techniques for the characterization of Vitis genotypes is suggested. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-2369 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 1992 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-23692024-09-06T18:29:42Z Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes Caracterización de variedades y portainjertos de vid (Vitis vinifera L.) mediante isoenzimas de las raíces Altube, H. A. Cabello, F. Ortiz, J. M. ampelography chemotaxonomy variety of vine rootstock root analysis enzyme protein ampelografía quimiotaxonomía variedades de vid portaingertos raíz análisis enzimas proteínas Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), malate dehydrogenases (MDH), and peroxidases (PER) were separated using polyacrylamide gels and later stained with specific stains for the various isoenzyme systems. ACPH, CO, EST, and MDH were found to be the most adequate systems for characterization. The results of the study of the different systems and the grouping of the cultivars are presented. The use of isoenzymatic techniques for the characterization of Vitis genotypes is suggested. Se eligieron 21 cultivares de Vitis vinifera L. y tres portainjertos para la caracterización bioquímica basada en la separación electroforética de las isoenzimas de extractos de raíz. Los sistemas isoenzimáticos estudiados fueron: fosfatasas ácidas (ACPH), esterasas (EST), catecol oxidasas (CO), glutamato oxalacetato transaminasas (GOT), malato deshidrogenasas (MDH) y peroxidasas (PER). La separación de los extractos de raíz se llevó a cabo en gel de poliacrilamida, con posterior tinción específica para los distintos sistemas enzimáticos. ACPH, CO, EST y MDH fueron los sistemas más adecuados para la caracterización. Se presentan los resultados de los diferentes sistemas y la agrupación de cultivares. Se recomienda el uso de técnicas isoenzimáticas en los estudios de caracterización del género Vitis. Facultad de Ciencias Agropecuarias 1992-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/2369 10.31047/1668.298x.v9.n2.2369 AgriScientia; Vol. 9 No. 2 (1992); 21-29 AgriScientia; Vol. 9 Núm. 2 (1992); 21-29 1668-298X 10.31047/1668.298x.v9.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/2369/1313 Derechos de autor 1992 H. A. Altube, F. Cabello, J. M. Ortiz https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | ampelography chemotaxonomy variety of vine rootstock root analysis enzyme protein ampelografía quimiotaxonomía variedades de vid portaingertos raíz análisis enzimas proteínas Altube, H. A. Cabello, F. Ortiz, J. M. Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title | Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title_alt | Caracterización de variedades y portainjertos de vid (Vitis vinifera L.) mediante isoenzimas de las raíces |
| title_full | Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title_fullStr | Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title_full_unstemmed | Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title_short | Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes |
| title_sort | characterization of grapevine vitis vinifera l varieties and rootstocks by root isoenzymes |
| topic | ampelography chemotaxonomy variety of vine rootstock root analysis enzyme protein ampelografía quimiotaxonomía variedades de vid portaingertos raíz análisis enzimas proteínas |
| topic_facet | ampelography chemotaxonomy variety of vine rootstock root analysis enzyme protein ampelografía quimiotaxonomía variedades de vid portaingertos raíz análisis enzimas proteínas |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/2369 |
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