Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
2022 metrics. Journal Impact factor: 7.2
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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Society for Experimental Biology
2024
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| Online Access: | http://hdl.handle.net/11086/552668 https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.16907 https://doi.org/10.1111/tpj.16907 |
| _version_ | 1806012472942919680 |
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| author | Lescano López, Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena |
| author2 | https://orcid.org/0000-0002-1194-9508 |
| author_facet | https://orcid.org/0000-0002-1194-9508 Lescano López, Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena |
| author_sort | Lescano López, Ignacio |
| collection | Repositorio Digital Universitario |
| description | 2022 metrics. Journal Impact factor: 7.2 |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552668 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| publisher | Society for Experimental Biology |
| record_format | dspace |
| spelling | rdu-unc.5526682024-07-11T06:22:12Z Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds Lescano López, Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena https://orcid.org/0000-0002-1194-9508 https://orcid.org/0000-0003-2775-4155 https://orcid.org/0000-0002-2894-7744 https://orcid.org/0000-0001-7001-5898 Base excision repair DNA glycosylases MBD4L Seed ageing Seed germination DNA damage responses 2022 metrics. Journal Impact factor: 7.2 info:eu-repo/semantics/publishedVersion Fil: Lescano López, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Torres, José Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Cecchini, Nicolás Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Álvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Álvarez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination. info:eu-repo/semantics/publishedVersion Fil: Lescano López, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Torres, José Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Cecchini, Nicolás Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Fil: Álvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina. Fil: Álvarez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. 2024-07-10T23:04:25Z 2024-07-10T23:04:25Z 2024-06-04 article López, I. L., Torres, J. R., Cecchini, N. M., & Alvarez, M. E. (2024). Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds. The Plant journal: for cell and molecular biology. http://hdl.handle.net/11086/552668 1365-313X https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.16907 https://doi.org/10.1111/tpj.16907 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Society for Experimental Biology |
| spellingShingle | Base excision repair DNA glycosylases MBD4L Seed ageing Seed germination DNA damage responses Lescano López, Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_full | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_fullStr | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_full_unstemmed | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_short | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_sort | arabidopsis dna glycosylase mbd4l improves recovery of aged seeds |
| topic | Base excision repair DNA glycosylases MBD4L Seed ageing Seed germination DNA damage responses |
| url | http://hdl.handle.net/11086/552668 https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.16907 https://doi.org/10.1111/tpj.16907 |
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