Cryopreservation of an artificial human oral mucosa stroma. A viability
<font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1"> <p align="LEFT">The aim of this study was to evaluate the viability and biomechanical properties of artificial human oral</p> <p align="L...
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| Format: | publishedVersion |
| Language: | eng |
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2019
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| Online Access: | http://hdl.handle.net/11086/13452 |
| _version_ | 1801214774287007744 |
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| author | Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| author_facet | Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| author_sort | Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| collection | Repositorio Digital Universitario |
| description | <font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">
<p align="LEFT">The aim of this study was to evaluate the viability and biomechanical properties of artificial human oral</p>
<p align="LEFT">mucosa stroma (HOMS) subjected to cryopreservation with different cryoprotectant solutions.</p>
<p align="LEFT">Artificial HOMS based on a fibrin?agarose matrix with human gingival fibroblasts cultured 7 days</p>
<p align="LEFT">in vitro were cryopreserved with three cryoprotectant solutions: (A) TC-199 Medium, DMSO 15%, albumin;</p>
<p align="LEFT">(B) DMEM, FCS, DMSO 10%; (C) QC Medium, glycerol. As controls, artificial HOMS not subjected to</p>
<p align="LEFT">cryopreservation (CF) and HOMS cryopreserved without cryoprotectant solution (CS) were used. Histological</p>
<p align="LEFT">analysis by light microscopy showed that solutions A and B preserved a pattern of porosity similar</p>
<p align="LEFT">to values in CF. Based on the number of intact cells in the fibrin?agarose matrix, substitutes preserved</p>
<p align="LEFT">with solution B showed the best results. Cell proliferation detected with PCNA immunochemical methods</p>
<p align="LEFT">showed that the cell proliferation index was highest in substitutes cryopreserved with solution B. The</p></font></font>
<p align="LEFT"><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">reculture method and cell viability analyses with Live & Dead</font></font><font face="AdvPSSym" size="1"><font face="AdvPSSym" size="1"> </font></font><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">revealed increased number of viable in</font></font></p><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">
<p align="LEFT">cells preserved with solution B. Artificial stroma substitutes in CS control samples showed the greatest</p>
<p align="LEFT">alterations in microstructure and cell proliferation. Analysis of the biomechanical properties showed that</p>
<p align="LEFT">substitutes cryopreserved with different solutions had adequate rheological parameters (yield stress,</p>
<p align="LEFT">elastic modulus and viscous modulus) and were therefore suitable for use in regenerative medicine.</p>
<p align="LEFT">These results establish effective methods of cryopreservation for all experimental situations and suggest</p>
<p align="LEFT">that solution B (DMEM, FCS, DMSO 10%) was the best cryoprotectant for the cryopreservation of an artificial</p>
<p>oral human mucosa substitute based on a fibrin?agarose matrix.</p></font></font> |
| format | publishedVersion |
| id | rdu-unc.13452 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2019 |
| record_format | dspace |
| spelling | rdu-unc.134522022-06-09T13:04:59Z Cryopreservation of an artificial human oral mucosa stroma. A viability Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX publishedVersion <font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1"> <p align="LEFT">The aim of this study was to evaluate the viability and biomechanical properties of artificial human oral</p> <p align="LEFT">mucosa stroma (HOMS) subjected to cryopreservation with different cryoprotectant solutions.</p> <p align="LEFT">Artificial HOMS based on a fibrin?agarose matrix with human gingival fibroblasts cultured 7 days</p> <p align="LEFT">in vitro were cryopreserved with three cryoprotectant solutions: (A) TC-199 Medium, DMSO 15%, albumin;</p> <p align="LEFT">(B) DMEM, FCS, DMSO 10%; (C) QC Medium, glycerol. As controls, artificial HOMS not subjected to</p> <p align="LEFT">cryopreservation (CF) and HOMS cryopreserved without cryoprotectant solution (CS) were used. Histological</p> <p align="LEFT">analysis by light microscopy showed that solutions A and B preserved a pattern of porosity similar</p> <p align="LEFT">to values in CF. Based on the number of intact cells in the fibrin?agarose matrix, substitutes preserved</p> <p align="LEFT">with solution B showed the best results. Cell proliferation detected with PCNA immunochemical methods</p> <p align="LEFT">showed that the cell proliferation index was highest in substitutes cryopreserved with solution B. The</p></font></font> <p align="LEFT"><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">reculture method and cell viability analyses with Live & Dead</font></font><font face="AdvPSSym" size="1"><font face="AdvPSSym" size="1"> </font></font><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">revealed increased number of viable in</font></font></p><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1"> <p align="LEFT">cells preserved with solution B. Artificial stroma substitutes in CS control samples showed the greatest</p> <p align="LEFT">alterations in microstructure and cell proliferation. Analysis of the biomechanical properties showed that</p> <p align="LEFT">substitutes cryopreserved with different solutions had adequate rheological parameters (yield stress,</p> <p align="LEFT">elastic modulus and viscous modulus) and were therefore suitable for use in regenerative medicine.</p> <p align="LEFT">These results establish effective methods of cryopreservation for all experimental situations and suggest</p> <p align="LEFT">that solution B (DMEM, FCS, DMSO 10%) was the best cryoprotectant for the cryopreservation of an artificial</p> <p>oral human mucosa substitute based on a fibrin?agarose matrix.</p></font></font> www.ncbi.nlm.nih.gov/pubmed/24177233 publishedVersion Otras Ciencias de la Salud 2019-10-29T16:05:40Z 2019-10-29T16:05:40Z 2013 article http://hdl.handle.net/11086/13452 eng Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso; Electrónico y/o Digital |
| spellingShingle | CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title | Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_full | Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_fullStr | Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_full_unstemmed | Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_short | Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_sort | cryopreservation of an artificial human oral mucosa stroma a viability |
| topic | CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX |
| url | http://hdl.handle.net/11086/13452 |
| work_keys_str_mv | AT marioarodriguezmodestotlopezlopezjuandgduranmiguelalaminosantoniocamposismaelarodriguez cryopreservationofanartificialhumanoralmucosastromaaviability |