Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato
Quality and performance in potato production systems is seriously affected by fungi diseases such as R. solani, whose chemical control is inefficient. Theobjective of the present work was to select isolates of Trichoderma spp. in vitro as potential biofungicides for the control of Rhizoctonia solani...
| Main Authors: | , , , , , |
|---|---|
| Format: | Online |
| Language: | spa |
| Published: |
Facultad de Ciencias Agropecuarias
2020
|
| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/29419 |
| _version_ | 1839026530847555584 |
|---|---|
| author | Pérez, Alejandro Andrés Perez, M. A. Martinez Coca, B. Rollhaiser, I. N. Blengini , M. C. B. |
| author_facet | Pérez, Alejandro Andrés Perez, M. A. Martinez Coca, B. Rollhaiser, I. N. Blengini , M. C. B. |
| author_sort | Pérez, Alejandro Andrés |
| collection | Portal de Revistas |
| description | Quality and performance in potato production systems is seriously affected by fungi diseases such as R. solani, whose chemical control is inefficient. Theobjective of the present work was to select isolates of Trichoderma spp. in vitro as potential biofungicides for the control of Rhizoctonia solani on potato.From soil samples from Calamuchita, Río Primero, Punilla and San Javier departments in the Province of Córdoba (Argentina), 18 native Trichodermastrains were isolated and genetically identified. They were evaluated in vitro by the dual culture method on their competence for the substrate, mycoparasitism and antibiosis capacity. It was possible to identify a group of five promising strains: T. atroviride ACp8 y CBa3, T. konigiopsis CBk2 y CBk4 y T. harzianum CBh2. These strains had more than 85 % overgrowth over the pathogen, were very competitive for the substrate (Grade 1) and had an antibiosis capacity higher than 35 % which inhibited radial growth through at least four mechanisms of hyphal interaction against R. solani. The selected strains that specifically recognize the phytopathogen and due to their native condition present highedaphoclimatic adaptation, may be incorporated into crop managementimproving the system sustainability. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-29419 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2020 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-294192023-04-13T17:14:00Z Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato Selección de aislamientos de Trichoderma spp. in vitro como potenciales biofungicidas para el control de Rhizoctonia solani Kühn en la papa Pérez, Alejandro Andrés Perez, M. A. Martinez Coca, B. Rollhaiser, I. N. Blengini , M. C. B. Biological Control infection action mechanisms rhizoctoniasis infección Rhizoctoniasis control biológico mecanismos de acción Quality and performance in potato production systems is seriously affected by fungi diseases such as R. solani, whose chemical control is inefficient. Theobjective of the present work was to select isolates of Trichoderma spp. in vitro as potential biofungicides for the control of Rhizoctonia solani on potato.From soil samples from Calamuchita, Río Primero, Punilla and San Javier departments in the Province of Córdoba (Argentina), 18 native Trichodermastrains were isolated and genetically identified. They were evaluated in vitro by the dual culture method on their competence for the substrate, mycoparasitism and antibiosis capacity. It was possible to identify a group of five promising strains: T. atroviride ACp8 y CBa3, T. konigiopsis CBk2 y CBk4 y T. harzianum CBh2. These strains had more than 85 % overgrowth over the pathogen, were very competitive for the substrate (Grade 1) and had an antibiosis capacity higher than 35 % which inhibited radial growth through at least four mechanisms of hyphal interaction against R. solani. The selected strains that specifically recognize the phytopathogen and due to their native condition present highedaphoclimatic adaptation, may be incorporated into crop managementimproving the system sustainability. La calidad y rendimiento en los sistemas de producción de papa se ve seriamente afectado por enfermedades por hongos como Rhizoctonia solani, cuyo control químico es ineficiente. El objetivo de este trabajo fue seleccionar aislamientos de Trichoderma spp. in vitro como potenciales biofungicidas para el control de R. solani en papa. A partir de muestras de suelo de los departamentos Calamuchita, Río Primero, Punilla y San Javier, de la provincia de Córdoba (Argentina), se aislaron e identificaron genéticamente 18 cepas nativas de Trichoderma. A través de cultivos duales in vitro se evaluó la competencia por el sustrato, micoparasitismo y capacidad de antibiosis. Se identificaron cinco cepas promisorias: T. atroviride ACp8 y CBa3, T. konigiopsis CBk2 y CBk4 y T. harzianum CBh2. Estas cepas presentaron un sobrecrecimiento mayor al 85 % sobre el patógeno; fueron muy competitivas por el sustrato (Grado 1) y presentaron una elevada capacidad de antibiosis mayor al 35 % lo que inhibió el crecimiento radial por al menos cuatro mecanismos de interacción hifal frente a R. solani. Las cepas seleccionadas que reconocen específicamente al fitopatógeno y presentan alta adaptación edafoclimática por su condición de nativas, podrán ser incorporadas al manejo del cultivo favoreciendo la sustentabilidad del sistema. Facultad de Ciencias Agropecuarias 2020-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/29419 10.31047/1668.298x.v37.n2.29419 AgriScientia; Vol. 37 No. 2 (2020); 21-33 AgriScientia; Vol. 37 Núm. 2 (2020); 21-33 1668-298X 10.31047/1668.298x.v37.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/29419/41043 https://revistas.unc.edu.ar/index.php/agris/article/view/29419/32703 Derechos de autor 2020 Alejandro Andrés Pérez, M. A. Perez, B. Martinez Coca, I. N. Rollhaiser, M. C. Blengini https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | Biological Control infection action mechanisms rhizoctoniasis infección Rhizoctoniasis control biológico mecanismos de acción Pérez, Alejandro Andrés Perez, M. A. Martinez Coca, B. Rollhaiser, I. N. Blengini , M. C. B. Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title | Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title_alt | Selección de aislamientos de Trichoderma spp. in vitro como potenciales biofungicidas para el control de Rhizoctonia solani Kühn en la papa |
| title_full | Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title_fullStr | Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title_full_unstemmed | Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title_short | Selection of Trichoderma spp. isolates in vitro as potential biofungicides for the control of Rhizoctonia solani Kühn on potato |
| title_sort | selection of trichoderma spp isolates in vitro as potential biofungicides for the control of rhizoctonia solani kuhn on potato |
| topic | Biological Control infection action mechanisms rhizoctoniasis infección Rhizoctoniasis control biológico mecanismos de acción |
| topic_facet | Biological Control infection action mechanisms rhizoctoniasis infección Rhizoctoniasis control biológico mecanismos de acción |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/29419 |
| work_keys_str_mv | AT perezalejandroandres selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT perezma selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT martinezcocab selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT rollhaiserin selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT blenginimc selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT b selectionoftrichodermasppisolatesinvitroaspotentialbiofungicidesforthecontrolofrhizoctoniasolanikuhnonpotato AT perezalejandroandres selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa AT perezma selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa AT martinezcocab selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa AT rollhaiserin selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa AT blenginimc selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa AT b selecciondeaislamientosdetrichodermasppinvitrocomopotencialesbiofungicidasparaelcontrolderhizoctoniasolanikuhnenlapapa |