Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/l + BA 2.25 mg/l...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | spa |
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Facultad de Ciencias Agropecuarias
1992
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/2941 |
| _version_ | 1842035301416960000 |
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| author | Zorzoli, R. Cointry, E. L. Prado, E. A. Mroginski, L. A. Picardi, L. A. |
| author_facet | Zorzoli, R. Cointry, E. L. Prado, E. A. Mroginski, L. A. Picardi, L. A. |
| author_sort | Zorzoli, R. |
| collection | Portal de Revistas |
| description | Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/l + BA 2.25 mg/l (M₁) and with BA 1.125 mg/l (M₁₁). Shoot-forming capacity was evaluated through two measures: the development rate (DR): proportion of explants with calli and/or shoots, and prolificity rate (Pr): proportion of shoots to developed explants. No differences among cultivars were found in both media for DR and Pr. In M₁, significant differences between reciprocal crosses (R × U – U × R) were found for Pr (t = 1.8; p < 0.05). F₂ plants were obtained by selfing the F₁ and 171 of the former had the U cytoplasm while 85 had the R one. The U cytoplasm Pr was 2.43 and R was 1.93 (t = 2.81; p < 0.01). These results would indicate that the cytoplasm effects on regeneration are still active in the F₂ generation. Since it can be inferred that the genetic variability for the regeneration ability might be inhibited by cytoplasmic effects. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-2941 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 1992 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-29412025-06-23T15:34:05Z Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) Influencia del citoplasma sobre la capacidad de regeneración in vitro en híbridos y sus generaciones segregantes en tomate (Lycopersicon esculentum Mill) Zorzoli, R. Cointry, E. L. Prado, E. A. Mroginski, L. A. Picardi, L. A. tomato hybrids shoot-forming capacity cytoplasm tomates híbridos capacidad de regeneración citoplasma Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/l + BA 2.25 mg/l (M₁) and with BA 1.125 mg/l (M₁₁). Shoot-forming capacity was evaluated through two measures: the development rate (DR): proportion of explants with calli and/or shoots, and prolificity rate (Pr): proportion of shoots to developed explants. No differences among cultivars were found in both media for DR and Pr. In M₁, significant differences between reciprocal crosses (R × U – U × R) were found for Pr (t = 1.8; p < 0.05). F₂ plants were obtained by selfing the F₁ and 171 of the former had the U cytoplasm while 85 had the R one. The U cytoplasm Pr was 2.43 and R was 1.93 (t = 2.81; p < 0.01). These results would indicate that the cytoplasm effects on regeneration are still active in the F₂ generation. Since it can be inferred that the genetic variability for the regeneration ability might be inhibited by cytoplasmic effects. Con el objeto de estudiar la influencia del citoplasma sobre la capacidad de regeneración in vitro en tomate se usaron tres cultivares de tomate (Rossol Selección La Consulta (R); UC 82 (U) y Platauco INTA (P), sus cruzas recíprocas y las F₂. Los explantos foliares se cultivaron sobre el medio Murashige y Skoog (MS) con 0,175 mg/l de AIA y 2,25 mg/l de BA (MI) y 1,125 mg/l de BA (MII). La capacidad de regeneración fue evaluada a través de 2 variables: la tasa de desarrollo (TD): proporción de explantos con callos y/o vástagos y la tasa de prolificidad (TP): proporción de vástagos por explantos desarrollados. No se encontraron diferencias en los cultivares entre ambos medios para TD ni para TP. En el MI se manifestaron diferencias para TP entre las cruzas recíprocas (R × U y U × R) (t = 1,8; p < 0,05). Las plantas F₂ se obtuvieron por autofecundación de las F₁. En la generación segregante las plantas con el citoplasma U presentaron una TP = 2,43 y para el citoplasma R = 1,93 (t = 2,81; p < 0,01).Estos resultados permiten concluir que los efectos del citoplasma encontrados en la F₁ se conservan enla F₂. Facultad de Ciencias Agropecuarias 1992-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/2941 10.31047/1668.298x.v9.n2.2941 AgriScientia; Vol. 9 No. 2 (1992); 15-19 AgriScientia; Vol. 9 Núm. 2 (1992); 15-19 1668-298X 10.31047/1668.298x.v9.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/2941/2767 Derechos de autor 1992 R. Zorzoli, E.L. Cointry, E.A. Prado, L.A. Mroginski, L.A. Picardi. https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | tomato hybrids shoot-forming capacity cytoplasm tomates híbridos capacidad de regeneración citoplasma Zorzoli, R. Cointry, E. L. Prado, E. A. Mroginski, L. A. Picardi, L. A. Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title | Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title_alt | Influencia del citoplasma sobre la capacidad de regeneración in vitro en híbridos y sus generaciones segregantes en tomate (Lycopersicon esculentum Mill) |
| title_full | Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title_fullStr | Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title_full_unstemmed | Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title_short | Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) |
| title_sort | influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato lycopersicon esculentum mill |
| topic | tomato hybrids shoot-forming capacity cytoplasm tomates híbridos capacidad de regeneración citoplasma |
| topic_facet | tomato hybrids shoot-forming capacity cytoplasm tomates híbridos capacidad de regeneración citoplasma |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/2941 |
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