Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells
Human pregnancy-specific glycoproteins (PSGs), encoded by eleven highly conserved genes, are the major placental polypeptides. Low PSG levels in maternal circulation have been associated with complicated pregnancies. However, expression of each PSG gene and their regulation during cytotrophoblast ce...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Elsevier
2010
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| Online Access: | http://hdl.handle.net/11336/187192 https://suquia.ffyh.unc.edu.ar/handle/suquia/175355 |
| _version_ | 1826515255161782272 |
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| author | Camolotto, Soledad Andrea Racca, Ana Cristina Rena, Viviana Celeste del Valle Nores, Rodrigo Patrito, Luis Clemente Genti de Raimondi, Susana Panzetta-Dutari, Graciela Maria del Valle |
| author_facet | Camolotto, Soledad Andrea Racca, Ana Cristina Rena, Viviana Celeste del Valle Nores, Rodrigo Patrito, Luis Clemente Genti de Raimondi, Susana Panzetta-Dutari, Graciela Maria del Valle |
| author_sort | Camolotto, Soledad Andrea |
| collection | Repositorio Suquia |
| description | Human pregnancy-specific glycoproteins (PSGs), encoded by eleven highly conserved genes, are the major placental polypeptides. Low PSG levels in maternal circulation have been associated with complicated pregnancies. However, expression of each PSG gene and their regulation during cytotrophoblast cell differentiation remain poorly explored. Herein, we analyze the expression of five PSG genes and demonstrate that they are almost undetectable in undifferentiated trophoblast, but are all transcribed in differentiated cells. Among them, PSG1, PSG3 and PSG5 genes achieve high mRNA levels while PSG7 and PSG9 are poorly expressed. In addition, total PSG proteins and transcripts markedly increase during trophoblast differentiation, preceding morphological syncytialization and βhCG expression. The 5′ regulatory region contributes to the transcriptional control of PSG gene induction in trophoblast cells undergoing differentiation. This responsive region in PSG3 maps within a 130 bp promoter sequence, which overlaps the transcription start site and requires a functional Retinoic Acid Responsive Element (RARE) and a GA-binding protein (GABP) consensus site for basal and differentiation-dependent promoter activity, respectively. Present findings provide novel data for understanding the control of PSG gene expression and demonstrate that their proteins and transcripts represent early markers of trophoblast differentiation. |
| format | info:eu-repo/semantics/article |
| id | suquia-ffyh.175355 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | suquia-ffyh.1753552024-08-02T01:23:19Z Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells Camolotto, Soledad Andrea Racca, Ana Cristina Rena, Viviana Celeste del Valle Nores, Rodrigo Patrito, Luis Clemente Genti de Raimondi, Susana Panzetta-Dutari, Graciela Maria del Valle Transcription Gene family PSG Trophoblast differentiation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Human pregnancy-specific glycoproteins (PSGs), encoded by eleven highly conserved genes, are the major placental polypeptides. Low PSG levels in maternal circulation have been associated with complicated pregnancies. However, expression of each PSG gene and their regulation during cytotrophoblast cell differentiation remain poorly explored. Herein, we analyze the expression of five PSG genes and demonstrate that they are almost undetectable in undifferentiated trophoblast, but are all transcribed in differentiated cells. Among them, PSG1, PSG3 and PSG5 genes achieve high mRNA levels while PSG7 and PSG9 are poorly expressed. In addition, total PSG proteins and transcripts markedly increase during trophoblast differentiation, preceding morphological syncytialization and βhCG expression. The 5′ regulatory region contributes to the transcriptional control of PSG gene induction in trophoblast cells undergoing differentiation. This responsive region in PSG3 maps within a 130 bp promoter sequence, which overlaps the transcription start site and requires a functional Retinoic Acid Responsive Element (RARE) and a GA-binding protein (GABP) consensus site for basal and differentiation-dependent promoter activity, respectively. Present findings provide novel data for understanding the control of PSG gene expression and demonstrate that their proteins and transcripts represent early markers of trophoblast differentiation. Fil: Camolotto, Soledad Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Racca, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Rena, Viviana Celeste del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Nores, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Patrito, Luis Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Genti de Raimondi, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Panzetta-Dutari, Graciela Maria del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina 2010-04 2024-08-02T01:23:19Z 2024-08-02T01:23:19Z info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/11336/187192 Camolotto, Soledad Andrea; Racca, Ana Cristina; Rena, Viviana Celeste del Valle; Nores, Rodrigo; Patrito, Luis Clemente; et al.; Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells; Elsevier; Placenta; 31; 4; 4-2010; 312-319 0143-4004 1532-3102 CONICET Digital CONICET https://suquia.ffyh.unc.edu.ar/handle/suquia/175355 eng info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0143400410000202 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2010.01.004 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf Elsevier |
| spellingShingle | Transcription Gene family PSG Trophoblast differentiation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Camolotto, Soledad Andrea Racca, Ana Cristina Rena, Viviana Celeste del Valle Nores, Rodrigo Patrito, Luis Clemente Genti de Raimondi, Susana Panzetta-Dutari, Graciela Maria del Valle Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title | Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title_full | Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title_fullStr | Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title_full_unstemmed | Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title_short | Expression and Transcriptional Regulation of Individual Pregnancy-specific Glycoprotein Genes in Differentiating Trophoblast Cells |
| title_sort | expression and transcriptional regulation of individual pregnancy specific glycoprotein genes in differentiating trophoblast cells |
| topic | Transcription Gene family PSG Trophoblast differentiation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/187192 https://suquia.ffyh.unc.edu.ar/handle/suquia/175355 |
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