Validation of a immunoassay for the determination of vancomycin in cerebospinal fluid.

Introduction: Vancomycin (VAN) is an antibiotic used to treat serious infections. Its use is related to adverse effects such as acute facial hyperemia, nephrotoxicity and ototoxicity. By having a very narrow therapeutic range, its monitoring is necessary to maximize efficiency and minimize toxic eff...

Full description

Found in: Portal de Revistas
Main Authors: Marks, Lucrecia; Hospital de Niños de la Santísima Trinidad, Laboratorio. Córdoba, Argentina., Duchein, Solange; Fundación Lennox, Laboratorio. Córdoba, Argentina., González, Isabel Inés; Hospital de Niños de la Santísima Trinidad, Laboratorio. Córdoba, Argentina. Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Farmacología. Córdoba, Argentina., Suárez, Hector Andrés; Hospital de Niños de la Santísima Trinidad, Laboratorio. Córdoba, Argentina. Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Farmacología. Córdoba, Argentina., Rivolta, Susana; Hospital de Niños de la Santísima Trinidad, Laboratorio. Córdoba, Argentina. Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Escuela de Salud Pública. Córdoba, Argentina.
Format: Online
Language: spa
Published: Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019
Online Access: https://revistas.unc.edu.ar/index.php/med/article/view/23550
Summary: Introduction: Vancomycin (VAN) is an antibiotic used to treat serious infections. Its use is related to adverse effects such as acute facial hyperemia, nephrotoxicity and ototoxicity. By having a very narrow therapeutic range, its monitoring is necessary to maximize efficiency and minimize toxic effects. It is estimated that its concentration in CSF is approximately 10% of the plasma level in patients who receive intravenous treatment and who have meninges inflammation. Plasma concentrations of VAN are not a reliable indicator of those present in CSF. The aim of this study was to validate an immunological method based on the kinetic interaction of microparticles in solution (KIMS) for the determination of VAN in CSF. Materials and Methods: KIMS was validated for the evaluation of VAN in CSF. For this, the parameters of linearity, precision, accuracy, limit of detection, limit of quantification, interference, selectivity and specificity were determined. Results: The method was linear in a range between 0 and 15 µg/mL, the CV% obtained oscillated between 0.7 and 2.5% on the linear range. The LOD and LOQ were 0.4 µg/mL and 1.4 µg/mL respectively. The equation of the line obtained based on the correlation of methods between KIMS and HPLC-UV was y = 0.9151x + 1.1695, R² = 0.9453. Conclusion: The KIMS method demonstrated to have an adequate sensitivity and specificity to determine VAN in CSF and being a useful tool for monitoring patients who present complicated infections at CNS level.