Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment
Impact Factor. 2023 (2024 update): 5.3
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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2024
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| Online Access: | http://hdl.handle.net/11086/553213 https://www.sciencedirect.com/science/article/abs/pii/S0378517324003326?via%3Dihub https://www.scopus.com/record/display.uri?eid=2-s2.0-85191178747&origin=inward&txGid=2e4211b9f2132817dba0230d9ebf6b5c https://pubmed.ncbi.nlm.nih.gov/38621614/ https://doi.org/10.1016/j.ijpharm.2024.124098 |
| _version_ | 1808112621978648576 |
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| author | Vallejo, Reinaldo Quinteros, Daniela Gutiérrez, Javier Martínez, Sofía Rodríguez Rojo, Soraya Tártara, Luis Ignacio Palma, Santiago Arias, Francisco Javier |
| author2 | https://orcid.org/0000-0001-7459-7442 |
| author_facet | https://orcid.org/0000-0001-7459-7442 Vallejo, Reinaldo Quinteros, Daniela Gutiérrez, Javier Martínez, Sofía Rodríguez Rojo, Soraya Tártara, Luis Ignacio Palma, Santiago Arias, Francisco Javier |
| author_sort | Vallejo, Reinaldo |
| collection | Repositorio Digital Universitario |
| description | Impact Factor. 2023 (2024 update): 5.3 |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.553213 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5532132024-08-09T06:24:42Z Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment Vallejo, Reinaldo Quinteros, Daniela Gutiérrez, Javier Martínez, Sofía Rodríguez Rojo, Soraya Tártara, Luis Ignacio Palma, Santiago Arias, Francisco Javier https://orcid.org/0000-0001-7459-7442 https://orcid.org/0000-0003-2767-9087 Acetazolamide Elastin-like recombinamers Nanoparticles Ophthalmic drug delivery Supercritical Antisolvent Impact Factor. 2023 (2024 update): 5.3 info:eu-repo/semantics/publishedVersion Fil: Vallejo, Reinaldo. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. Fil: Vallejo, Reinaldo. BioEcoUVa, Research Institute on Bioeconomy, High Pressure Process Group, University of Valladolid, Department of Chemical Engineering and Environmental Technology, Escuela de Ingenierías Industriales, Sede Mergelina; Spain. Fil: Quinteros, Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Quinteros, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Gutiérrez, Javier. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. Fil: Martínez, Sofía. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Martínez, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Rodríguez Rojo, Soraya. BioEcoUVa, Research Institute on Bioeconomy, High Pressure Process Group, University of Valladolid, Department of Chemical Engineering and Environmental Technology, Escuela de Ingenierías Industriales, Sede Mergelina; Spain. Fil: Tártara, Luis Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Tártara, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Palma, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Palma, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Arias, Francisco Javier. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. Glaucoma, the second most common cause of blindness worldwide, requires the development of new and effective treatments. This study introduces a novel controlled-release system utilizing elastin-like recombinamers (ELR) and the Supercritical Antisolvent (SAS) technique with supercritical CO2. Acetazolamide (AZM), a class IV drug with limited solubility and permeability, is successfully encapsulated in an amphiphilic ELR at three different ELR:AZM ratios, yielding up to 62 %. Scanning electron microscopy (SEM) reveals spherical microparticles that disintegrate into monodisperse nanoparticles measuring approximately 42 nm under physiological conditions. The nanoparticles, as observed via Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), do not exhibit aggregates, a fact confirmed by the zeta potential displaying a value of –33 mV over a period of 30 days. Transcorneal permeation tests demonstrate a 10 % higher permeation level compared to the control solution, which increases to 30 % after 2 h. Ocular irritation tests demonstrate no adverse effects or damage. Intraocular pressure (IOP) tests conducted on hypertensive rabbits indicate greater effectiveness for all three analyzed formulations, suggesting enhanced drug bioavailability during treatment. Consequently, the combination of recombinant biopolymers and high-pressure techniques represents a promising approach for advancing glaucoma therapy, emphasizing its potential clinical significance. info:eu-repo/semantics/publishedVersion Fil: Vallejo, Reinaldo. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. Fil: Vallejo, Reinaldo. BioEcoUVa, Research Institute on Bioeconomy, High Pressure Process Group, University of Valladolid, Department of Chemical Engineering and Environmental Technology, Escuela de Ingenierías Industriales, Sede Mergelina; Spain. Fil: Quinteros, Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Quinteros, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Gutiérrez, Javier. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. Fil: Martínez, Sofía. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Martínez, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Rodríguez Rojo, Soraya. BioEcoUVa, Research Institute on Bioeconomy, High Pressure Process Group, University of Valladolid, Department of Chemical Engineering and Environmental Technology, Escuela de Ingenierías Industriales, Sede Mergelina; Spain. Fil: Tártara, Luis Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Tártara, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Palma, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Fil: Palma, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica, Ciudad Universitaria; Argentina. Fil: Arias, Francisco Javier. Smart Devices for Nano Medicine Group, Unidad Excelencia Instituto de BioMedicina y Genética Molecular (IBGM) de Valladolid, University of Valladolid and CSIC, Valladolid; Spain. 2024-08-08T17:26:38Z 2024-08-08T17:26:38Z 2024-05-25 article Vallejo, R., Quinteros, D., Gutiérrez, J., Martínez, S., Rojo, S. R., Tártara, L. I., ... & Arias, F. J. (2024). Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment. International Journal of Pharmaceutics, 657, 124098. http://hdl.handle.net/11086/553213 1873-3476 https://www.sciencedirect.com/science/article/abs/pii/S0378517324003326?via%3Dihub https://www.scopus.com/record/display.uri?eid=2-s2.0-85191178747&origin=inward&txGid=2e4211b9f2132817dba0230d9ebf6b5c https://pubmed.ncbi.nlm.nih.gov/38621614/ https://doi.org/10.1016/j.ijpharm.2024.124098 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spellingShingle | Acetazolamide Elastin-like recombinamers Nanoparticles Ophthalmic drug delivery Supercritical Antisolvent Vallejo, Reinaldo Quinteros, Daniela Gutiérrez, Javier Martínez, Sofía Rodríguez Rojo, Soraya Tártara, Luis Ignacio Palma, Santiago Arias, Francisco Javier Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title | Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title_full | Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title_fullStr | Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title_full_unstemmed | Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title_short | Acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent (SAS) process for glaucoma treatment |
| title_sort | acetazolamide encapsulation in elastin like recombinamers using a supercritical antisolvent sas process for glaucoma treatment |
| topic | Acetazolamide Elastin-like recombinamers Nanoparticles Ophthalmic drug delivery Supercritical Antisolvent |
| url | http://hdl.handle.net/11086/553213 https://www.sciencedirect.com/science/article/abs/pii/S0378517324003326?via%3Dihub https://www.scopus.com/record/display.uri?eid=2-s2.0-85191178747&origin=inward&txGid=2e4211b9f2132817dba0230d9ebf6b5c https://pubmed.ncbi.nlm.nih.gov/38621614/ https://doi.org/10.1016/j.ijpharm.2024.124098 |
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