Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population
Fil: Marozzi, Antonela Alejandra. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina.
| Main Authors: | , , , , , , , , |
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
| Published: |
2024
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| Online Access: | http://hdl.handle.net/11086/552638 https://academic.oup.com/conphys/issue/12/1 http://doi/10.1093/conphys/coae003 |
| _version_ | 1806012319490113536 |
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| author | Marozzi, Antonela Alejandra Cantarelli, Verónica I. Panebianco, Antonella Gomez, F. M. Ovejero, Ramiro Gregorio, P. F. Peña, F. Ponzio, Marina F. Carmanchahi, Pablo Daniel |
| author2 | https://orcid.org/0000-0001-7382-8413 |
| author_facet | https://orcid.org/0000-0001-7382-8413 Marozzi, Antonela Alejandra Cantarelli, Verónica I. Panebianco, Antonella Gomez, F. M. Ovejero, Ramiro Gregorio, P. F. Peña, F. Ponzio, Marina F. Carmanchahi, Pablo Daniel |
| author_sort | Marozzi, Antonela Alejandra |
| collection | Repositorio Digital Universitario |
| description | Fil: Marozzi, Antonela Alejandra. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552638 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5526382024-07-09T06:22:18Z Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population Marozzi, Antonela Alejandra Cantarelli, Verónica I. Panebianco, Antonella Gomez, F. M. Ovejero, Ramiro Gregorio, P. F. Peña, F. Ponzio, Marina F. Carmanchahi, Pablo Daniel https://orcid.org/0000-0001-7382-8413 https://orcid.org/0000-0001-7900-1118 Estrone conjugates Gestation Pregnancy rate Pregnanediol glucuronides info:eu-repo/semantics/publishedVersion Fil: Marozzi, Antonela Alejandra. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Cantarelli, Verónica I. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Cantarelli, Verónica I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Panebianco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Gomez, F. M. Universidad Nacional del Comahue. Asentamiento Universitario San Martín de los Andes; Argentina. Fil: Ovejero, Ramiro. Universidad Nacional de Tucumán; Argentina. Fil: Ovejero, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ecología Regional; Argentina. Fil: Gregorio, P. F. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Gregorio, P. F. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Peña, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Witral - Red de Investigaciones en conservación y manejo de vida silvestre en sistemas socio-ecológicos, Instituto Argentino de Investigaciones de Zonas Áridas; Argentina. Fil: Ponzio, Marina F. Universidad Nacional del Comahue. Asentamiento Universitario San Martín de los Andes; Argentina. Fil: Carmanchahi, Pablo Daniel. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is a widely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiological information without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate a non-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe) and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through the gestation period (∼12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormones was detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of the gestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal samples were collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation and at the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% of the population; at the end of gestation, only 20–40% of the females had confirmed pregnancies. Our results demonstrated that the polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted through feces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, the findings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Although some abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestational process may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductive physiology of one of the most emblematic ungulates in South America. info:eu-repo/semantics/publishedVersion Fil: Marozzi, Antonela Alejandra. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Cantarelli, Verónica I. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Cantarelli, Verónica I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Panebianco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Gomez, F. M. Universidad Nacional del Comahue. Asentamiento Universitario San Martín de los Andes; Argentina. Fil: Ovejero, Ramiro. Universidad Nacional de Tucumán; Argentina. Fil: Ovejero, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ecología Regional; Argentina. Fil: Gregorio, P. F. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Gregorio, P. F. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Fil: Peña, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Witral - Red de Investigaciones en conservación y manejo de vida silvestre en sistemas socio-ecológicos, Instituto Argentino de Investigaciones de Zonas Áridas; Argentina. Fil: Ponzio, Marina F. Universidad Nacional del Comahue. Asentamiento Universitario San Martín de los Andes; Argentina. Fil: Carmanchahi, Pablo Daniel. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Fil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones en Ecofisiología de Fauna Silvestre. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. 2024-07-08T19:06:01Z 2024-07-08T19:06:01Z 2024-01-09 article Marozzi A, Cantarelli VI, Panebianco A, Gomez FM, Ovejero R, Gregorio PF, Peña F, Ponzio MF, Carmanchahi PD (2024) Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population. Conserv Physiol 12(1): coae003; doi:10.1093/conphys/coae003. http://hdl.handle.net/11086/552638 2051-1434 https://academic.oup.com/conphys/issue/12/1 http://doi/10.1093/conphys/coae003 eng Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Estrone conjugates Gestation Pregnancy rate Pregnanediol glucuronides Marozzi, Antonela Alejandra Cantarelli, Verónica I. Panebianco, Antonella Gomez, F. M. Ovejero, Ramiro Gregorio, P. F. Peña, F. Ponzio, Marina F. Carmanchahi, Pablo Daniel Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title | Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title_full | Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title_fullStr | Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title_full_unstemmed | Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title_short | Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population |
| title_sort | non invasive assessment of hormonal fluctuations during pregnancy in guanacos lama guanicoe and its application in a wild population |
| topic | Estrone conjugates Gestation Pregnancy rate Pregnanediol glucuronides |
| url | http://hdl.handle.net/11086/552638 https://academic.oup.com/conphys/issue/12/1 http://doi/10.1093/conphys/coae003 |
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