Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols
Impact Factor: 4.9 (2023); 5-Year Impact Factor: 5.2 (2023).
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
| Published: |
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11086/552605 https://www.mdpi.com/2079-6374/14/6/278 https://doi.org/10.3390/bios14060278 |
| _version_ | 1806012160052035584 |
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| author | Piccoli, María Belén Gulotta, Florencia Alejandra Montenegro, Mariana Angélica Vanden Braber, Noelia Luciana Paz Zanini, Verónica Irene Ferreyra, Nancy Fabiana |
| author2 | https://orcid.org/0000-0002-0872-755X |
| author_facet | https://orcid.org/0000-0002-0872-755X Piccoli, María Belén Gulotta, Florencia Alejandra Montenegro, Mariana Angélica Vanden Braber, Noelia Luciana Paz Zanini, Verónica Irene Ferreyra, Nancy Fabiana |
| author_sort | Piccoli, María Belén |
| collection | Repositorio Digital Universitario |
| description | Impact Factor: 4.9 (2023); 5-Year Impact Factor: 5.2 (2023). |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552605 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5526052024-07-07T06:25:05Z Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols Piccoli, María Belén Gulotta, Florencia Alejandra Montenegro, Mariana Angélica Vanden Braber, Noelia Luciana Paz Zanini, Verónica Irene Ferreyra, Nancy Fabiana https://orcid.org/0000-0002-0872-755X https://orcid.org/0000-0002-3402-417X https://orcid.org/0000-0002-4180-975X Water soluble chitosan derivatives Glucosamine–chitosan Sodium montmorillonite Horseradish peroxidase Polyphenols Impact Factor: 4.9 (2023); 5-Year Impact Factor: 5.2 (2023). info:eu-repo/semantics/publishedVersion Fil: Piccoli, María Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica, Argentina. Fil: Piccoli, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Fisicoquímica de Córdoba, Argentina. Fil: Gulotta, Florencia Alejandra. Independent Researcher, Santiago del Estero, Argentina. Fil: Montenegro, Mariana Angélica. Universidad Nacional de Villa María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María, Argentina. Fil: Vanden Braber, Noelia Luciana. Universidad Nacional de Villa María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María, Argentina. Fil: Paz Zanini, Verónica Irene. Universidad Nacional de Santiago del Estero. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA, Argentina. Fil: Ferreyra, Nancy Fabiana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica, Argentina. Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Fisicoquímica de Córdoba, Argentina. Glucosamine–chitosan synthesized by the Maillard reaction was combined with montmorillonite to obtain a nanohybrid composite to immobilize horseradish peroxidase. The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay–polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 ± 0.2) × 102 µA mM−1 and (74 ± 8) nM; (1.2 ± 0.1) × 102 µA mM−1 and (26 ± 3) nM; (16 ± 2) µA mM−1 and (0.74 ± 0.09) µM; and (3.7± 0.3) µA mM−1 and (3.3 ± 0.2) µM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts. info:eu-repo/semantics/publishedVersion Fil: Piccoli, María Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica, Argentina. Fil: Piccoli, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Fisicoquímica de Córdoba, Argentina. Fil: Gulotta, Florencia Alejandra. Independent Researcher, Santiago del Estero, Argentina. Fil: Montenegro, Mariana Angélica. Universidad Nacional de Villa María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María, Argentina. Fil: Vanden Braber, Noelia Luciana. Universidad Nacional de Villa María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María, Argentina. Fil: Paz Zanini, Verónica Irene. Universidad Nacional de Santiago del Estero. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA, Argentina. Fil: Ferreyra, Nancy Fabiana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica, Argentina. Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Fisicoquímica de Córdoba, Argentina. 2024-07-07T02:12:01Z 2024-07-07T02:12:01Z 2024-05-29 article Piccoli, M. B., Gulotta, F. A., Montenegro, M. A., Vanden Braber, N. L., Paz Zanini, V. I., & Ferreyra, N. F. (2024). Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols. Biosensors, 14(6), 278. http://hdl.handle.net/11086/552605 2079-6374 https://www.mdpi.com/2079-6374/14/6/278 https://doi.org/10.3390/bios14060278 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spellingShingle | Water soluble chitosan derivatives Glucosamine–chitosan Sodium montmorillonite Horseradish peroxidase Polyphenols Piccoli, María Belén Gulotta, Florencia Alejandra Montenegro, Mariana Angélica Vanden Braber, Noelia Luciana Paz Zanini, Verónica Irene Ferreyra, Nancy Fabiana Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title | Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title_full | Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title_fullStr | Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title_full_unstemmed | Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title_short | Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols |
| title_sort | immobilization of horseradish peroxidase onto montmorillonite glucosamine chitosan composite for electrochemical biosensing of polyphenols |
| topic | Water soluble chitosan derivatives Glucosamine–chitosan Sodium montmorillonite Horseradish peroxidase Polyphenols |
| url | http://hdl.handle.net/11086/552605 https://www.mdpi.com/2079-6374/14/6/278 https://doi.org/10.3390/bios14060278 |
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