Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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2024
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| Online Access: | http://hdl.handle.net/11086/552568 https://www.mdpi.com/2218-1989/14/3/161 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/ https://doi.org/10.3390/metabo14030161 |
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| author | Schmitt, Luisa O. Blanco, Antonella Lima, Sheila V. Mancini, Gianni Mendes, Natalia F. Latini, Alexandra Gaspar, Joana M. |
| author2 | https://orcid.org/0000-0002-5127-5640 |
| author_facet | https://orcid.org/0000-0002-5127-5640 Schmitt, Luisa O. Blanco, Antonella Lima, Sheila V. Mancini, Gianni Mendes, Natalia F. Latini, Alexandra Gaspar, Joana M. |
| author_sort | Schmitt, Luisa O. |
| collection | Repositorio Digital Universitario |
| description | Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552568 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5525682024-07-05T06:26:34Z Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function Schmitt, Luisa O. Blanco, Antonella Lima, Sheila V. Mancini, Gianni Mendes, Natalia F. Latini, Alexandra Gaspar, Joana M. https://orcid.org/0000-0002-5127-5640 https://orcid.org/0000-0001-9032-1411 https://orcid.org/0000-0003-3151-4234 Obesity Inflammation Mitochondria Saturated fatty acids Astrocytes info:eu-repo/semantics/publishedVersion Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina. Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil. Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil. Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil. Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 μM and 400 μM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 μM palmitate decreased cell viability, and treatment with 200 μM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction. info:eu-repo/semantics/publishedVersion Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina. Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil. Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil. Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil. Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil. Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil. 2024-07-04T17:30:01Z 2024-07-04T17:30:01Z 2024-03-12 article Schmitt, L.O.; Blanco, A.; Lima, S.V.; Mancini, G.; Mendes, N.F.; Latini, A.; Gaspar, J.M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function. Metabolites 2024, 14, 161. https:// doi.org/10.3390/metabo14030161 http://hdl.handle.net/11086/552568 2218-1989 https://www.mdpi.com/2218-1989/14/3/161 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/ https://doi.org/10.3390/metabo14030161 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spellingShingle | Obesity Inflammation Mitochondria Saturated fatty acids Astrocytes Schmitt, Luisa O. Blanco, Antonella Lima, Sheila V. Mancini, Gianni Mendes, Natalia F. Latini, Alexandra Gaspar, Joana M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title | Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title_full | Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title_fullStr | Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title_full_unstemmed | Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title_short | Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function |
| title_sort | palmitate compromises c6 astrocytic cell viability and mitochondrial function |
| topic | Obesity Inflammation Mitochondria Saturated fatty acids Astrocytes |
| url | http://hdl.handle.net/11086/552568 https://www.mdpi.com/2218-1989/14/3/161 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/ https://doi.org/10.3390/metabo14030161 |
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