Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
2023 (2024 update): 5.0
| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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Association for Research in Vision and Ophthalmology.
2024
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| Online Access: | http://hdl.handle.net/11086/552548 https://iovs.arvojournals.org/article.aspx?articleid=2796328 |
| _version_ | 1806012314482114560 |
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| author | Vaglienti, María Victoria Subirada, Paula Virginia Paz, María Constanza Bonacci, Gustavo Sanchez, Maria Cecilia |
| author2 | https://orcid.org/0009-0007-0735-5356 |
| author_facet | https://orcid.org/0009-0007-0735-5356 Vaglienti, María Victoria Subirada, Paula Virginia Paz, María Constanza Bonacci, Gustavo Sanchez, Maria Cecilia |
| author_sort | Vaglienti, María Victoria |
| collection | Repositorio Digital Universitario |
| description | 2023 (2024 update): 5.0 |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552548 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| publisher | Association for Research in Vision and Ophthalmology. |
| record_format | dspace |
| spelling | rdu-unc.5525482024-07-08T06:22:18Z Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy Vaglienti, María Victoria Subirada, Paula Virginia Paz, María Constanza Bonacci, Gustavo Sanchez, Maria Cecilia https://orcid.org/0009-0007-0735-5356 https://orcid.org/0000-0003-3185-1951 https://orcid.org/0000-0002-1835-6713 https://orcid.org/0000-0002-9830-1850 https://orcid.org/0000-0003-4760-8518 2023 (2024 update): 5.0 info:eu-repo/semantics/publishedVersion Fil: Vaglienti, María Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Vaglienti, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Subirada, Paula Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Subirada, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Paz, María Constanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Paz, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Bonacci, Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Bonacci, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Sanchez, Maria Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Sanchez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Purpose : Inflammation, oxidative and nitrosative stress are involved in neovascular retinopathies (NR). Nitro-fatty acids are important electrophilic signaling mediators with anti-inflammatory, antioxidant and cytoprotective properties. Therefore, our aim was to evaluate the effect of nitro-oleic acid (NO2-OA) in the OIR mouse model. Methods : Briefly, C57BL/6 mice (n=35) were exposed to 75% O2 from postnatal day (P)7 to P12, after which they were brought to room air (RA) for additional 5 (P17) or 14 days (P26). Age-match mice maintained in RA (n=25) were used as controls. OIR mice were intraocular injected with 5μM of NO2-OA (n=22) or vehicle (n=13) at P12 and intraperitoneal at P14, P17, P20, P23 with 15mg/Kg of NO2-OA or vehicle. At P17 or P26 mice were sacrificed. Some eyes were fixed for whole mount staining of retina and microscopic analysis and other retinas were used for WB or RT-PCR assays. Retinal functionality was assessed at P17 or P26 by scotopic ERG in dark-adapted mice. Some eyes were fixed to obtain cryosections for TUNEL assay. GraphPad Prism8 was used for statistical analysis. Results : NO2-OA induced vascular regrowth (p<0.05) and reduced neovascularization (p<0.001) at P17 OIR. RT-PCR revealed a significant increase in VEGF levels in OIR mice respect to RA mice (p<0.0001), but not difference was found between NO2-OA treatment and vehicle (p=0.9978). Regarding PEDF, neither significant changes were observed in OIR respect to RA mice (p=0.1394) nor between NO2-OA and vehicle (p=0.1849). In addition, WB of neural retinas showed that NO2-OA prevented glial stress (p<0.0001) induced in OIR at P17 (p=0.3160). NO2-OA did not modify the decrease (p=0.0177) in GS at P17 (p=0.0003). The ERG analysis showed a significant decrease in b-wave amplitude in OIR compared to RA mice. Also, a- and b- wave latencies were significantly increased in OIR respect to RA. Besides, WB analysis of retinas from RA and OIR mice revealed decreased levels of caspase-3 protein at P26 OIR. Finally, quantitative analysis showed that TUNEL-positive cells were significantly higher in P26 OIR compared to RA (p<0.05). All of these pathological events were prevented by NO2-OA (p=0.1362) (p=0.0004) (p<0.05). Conclusions : In conclusion, our study underscores the role of NO2-OA as potential treatment in the pathogenesis of NR in order to attenuate vascular and non-vascular alterations. This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024. info:eu-repo/semantics/publishedVersion Fil: Vaglienti, María Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Vaglienti, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Subirada, Paula Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Subirada, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Paz, María Constanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Paz, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Bonacci, Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Bonacci, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Sanchez, Maria Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Sanchez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. 2024-07-04T12:18:56Z 2024-07-04T12:18:56Z 2024-06 article Vaglienti, M. V., Subirada, P. V., Paz, M. C., Bonacci, G., & Sanchez, M. C. (2024). Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy. Investigative Ophthalmology & Visual Science, 65(7), 270-270. http://hdl.handle.net/11086/552548 1552-5783 https://iovs.arvojournals.org/article.aspx?articleid=2796328 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Association for Research in Vision and Ophthalmology. |
| spellingShingle | Vaglienti, María Victoria Subirada, Paula Virginia Paz, María Constanza Bonacci, Gustavo Sanchez, Maria Cecilia Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title | Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title_full | Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title_fullStr | Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title_full_unstemmed | Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title_short | Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy |
| title_sort | protective effect of nitro oleic acids in neovascularization gliosis and neurodegeneration in a mouse model of oxygen induced retinopathy |
| url | http://hdl.handle.net/11086/552548 https://iovs.arvojournals.org/article.aspx?articleid=2796328 |
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