The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite
Factor de Impacto 4.9 Journal Rank: JCR - Q1 (Biochemistry and Molecular Biology)
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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2024
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| Online Access: | http://hdl.handle.net/11086/552375 https://www.mdpi.com/1422-0067/24/11/9559 https://doi.org/10.3390/ijms24119559 |
| _version_ | 1802926369078771712 |
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| author | Natali, Lautaro Pizarro, Gabriel Luna Moyano, Sofía de la Cruz-Thea, Benjamín Musso, Juliana Rópolo, Andrea S. Eichner, Norbert Meister, Gunter Musri, Melina M. Feliziani, Constanza Touz, María C. |
| author2 | https://orcid.org/0009-0002-9928-1748 |
| author_facet | https://orcid.org/0009-0002-9928-1748 Natali, Lautaro Pizarro, Gabriel Luna Moyano, Sofía de la Cruz-Thea, Benjamín Musso, Juliana Rópolo, Andrea S. Eichner, Norbert Meister, Gunter Musri, Melina M. Feliziani, Constanza Touz, María C. |
| author_sort | Natali, Lautaro |
| collection | Repositorio Digital Universitario |
| description | Factor de Impacto 4.9
Journal Rank: JCR - Q1 (Biochemistry and Molecular Biology) |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552375 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5523752024-06-25T06:39:32Z The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite Natali, Lautaro Pizarro, Gabriel Luna Moyano, Sofía de la Cruz-Thea, Benjamín Musso, Juliana Rópolo, Andrea S. Eichner, Norbert Meister, Gunter Musri, Melina M. Feliziani, Constanza Touz, María C. https://orcid.org/0009-0002-9928-1748 https://orcid.org/0009-0000-9131-9911 https://orcid.org/0000-0001-6800-6634 https://orcid.org/0000-0003-2045-706X https://orcid.org/0000-0002-9159-5497 https://orcid.org/0000-0002-7581-5816 https://orcid.org/0000-0002-6769-6896 Giardia lamblia Parasite to parasite communication Exosomal-like vesicles (ElVs) Small RNAs (sRNA) msRNA tsRNA rsRNA Trophozoites Host-specificity Pathogenesis Factor de Impacto 4.9 Journal Rank: JCR - Q1 (Biochemistry and Molecular Biology) info:eu-repo/semantics/publishedVersion Fil: Natali Lautaro. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Pizarro Gabriel Luna. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Sofía Moyano. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: de la Cruz-Thea Benjamín. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Musso, Juliana. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Rópolo Andrea S. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Eichner, Norbert. University of Regensburg. Regensburg Center for Biochemistry. Laboratory for RNA Biology, Regensburg, Germany. Fil: Meister Gunter. University of Regensburg. Regensburg Center for Biochemistry. Laboratory for RNA Biology, Regensburg, Germany. Fil: Musri Melina M. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Musri Melina M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Argentina. Fil: Feliziani, Constanza. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Touz María C. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. The Giardia assemblages are separated by large genetic distances, which might account for their relevant biological and pathogenic differences. In this work, we analyzed the RNAs cargo released into exosomal-like vesicles (ElVs) by the assemblages A and B, which differentially infect humans, and the assemblage E, which infects hoofed animals. The RNA sequencing analysis revealed that the ElVs of each assemblage contained distinct small RNA (sRNA) biotypes, suggesting a preference for specific packaging in each assemblage. These sRNAs were classified into three categories, ribosomal-small RNAs (rsRNAs), messenger-small RNAs (msRNAs), and transfer-small RNAs (tsRNAs), which may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis. Uptake experiments showed, for the first time, that ElVs were successfully internalized by the parasite trophozoites. Furthermore, we observed that the sRNAs contained inside these ElVs were first located below the plasma membrane but then distributed along the cytoplasm. Overall, the study provides new insights into the molecular mechanisms underlying the host-specificity and pathogenesis of G. lamblia and highlights the potential role of sRNAs in parasite communication and regulation. info:eu-repo/semantics/publishedVersion Fil: Natali Lautaro. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Pizarro Gabriel Luna. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Sofía Moyano. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: de la Cruz-Thea Benjamín. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Musso, Juliana. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Rópolo Andrea S. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Eichner, Norbert. University of Regensburg. Regensburg Center for Biochemistry. Laboratory for RNA Biology, Regensburg, Germany. Fil: Meister Gunter. University of Regensburg. Regensburg Center for Biochemistry. Laboratory for RNA Biology, Regensburg, Germany. Fil: Musri Melina M. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Musri Melina M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Argentina. Fil: Feliziani, Constanza. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. Fil: Touz María C. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra, Argentina. 2024-06-24T16:56:33Z 2024-06-24T16:56:33Z 2023-05-31 article Natali, L.; Luna Pizarro, G.; Moyano, S.; de la Cruz-Thea, B.; Musso, J.; Rópolo, A.S.; Eichner, N.; Meister, G.; Musri, M.M.; Feliziani, C.; et al. The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite. Int. J. Mol. Sci. 2023, 24, 9559. https://doi.org/10.3390/ijms24119559 http://hdl.handle.net/11086/552375 1422-0067 https://www.mdpi.com/1422-0067/24/11/9559 https://doi.org/10.3390/ijms24119559 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Giardia lamblia Parasite to parasite communication Exosomal-like vesicles (ElVs) Small RNAs (sRNA) msRNA tsRNA rsRNA Trophozoites Host-specificity Pathogenesis Natali, Lautaro Pizarro, Gabriel Luna Moyano, Sofía de la Cruz-Thea, Benjamín Musso, Juliana Rópolo, Andrea S. Eichner, Norbert Meister, Gunter Musri, Melina M. Feliziani, Constanza Touz, María C. The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title | The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title_full | The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title_fullStr | The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title_full_unstemmed | The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title_short | The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite |
| title_sort | exosome like vesicles of giardia assemblages a b and e are involved in the delivering of distinct small rna from parasite to parasite |
| topic | Giardia lamblia Parasite to parasite communication Exosomal-like vesicles (ElVs) Small RNAs (sRNA) msRNA tsRNA rsRNA Trophozoites Host-specificity Pathogenesis |
| url | http://hdl.handle.net/11086/552375 https://www.mdpi.com/1422-0067/24/11/9559 https://doi.org/10.3390/ijms24119559 |
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