Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases?
Fil: Socas, Luis B. P. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina.
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | spa |
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American Chemical Society
2024
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| Online Access: | http://hdl.handle.net/11086/552327 https://pubs.acs.org/doi/full/10.1021/jacs.4c03443 |
| _version_ | 1802926359416143872 |
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| author | Socas, Luis B. P. Valdivia Pérez, Jessica A. Fanani, María L. Ambroggio, Ernesto E. |
| author2 | https://orcid.org/0000-0003-2392-4663 |
| author_facet | https://orcid.org/0000-0003-2392-4663 Socas, Luis B. P. Valdivia Pérez, Jessica A. Fanani, María L. Ambroggio, Ernesto E. |
| author_sort | Socas, Luis B. P. |
| collection | Repositorio Digital Universitario |
| description | Fil: Socas, Luis B. P. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.552327 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2024 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | rdu-unc.5523272024-06-26T06:26:21Z Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? Socas, Luis B. P. Valdivia Pérez, Jessica A. Fanani, María L. Ambroggio, Ernesto E. https://orcid.org/0000-0003-2392-4663 http://orcid.org/0000-0003-2278-198X Chemical structure Interfaces Lipids Membranes Phase transitions info:eu-repo/semantics/publishedVersion Fil: Socas, Luis B. P. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Socas, Luis B. P. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Valdivia-Pérez Jessica A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Valdivia-Pérez Jessica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Fanani María L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Fanani María L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Ambroggio Ernesto E. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Ambroggio Ernesto E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. The impact of lipid diversity on the lateral organization of biological membranes remains a topic of debate. While the existence of domains in lamellar membranes is well-established, the nonlamellar phases occurring in biological systems are less explored due to technical constraints. Here, we present the measurement of the excitation–emission matrices (EEM) of LAURDAN in several lipid structures. LAURDAN is a fluorescence probe widely used for characterizing lipid assemblies. The EEMs were analyzed by multidimensional spectral phasors (MdSP), an approach that seizes information from both the excitation and emission spectra. We developed a computer algorithm to construct EEM data based on a model for LAURDAN’s photophysics. The MdSP calculated from the simulated EEMs reveals that all feasible possibilities lie inside a universal triangle in the phasor’s plot. We use this triangle to propose a ternary representation for the phasors, allowing a better assessment of LAURDAN’s surroundings in terms of hydration, water mobility, and local electronic environment. Building upon this foundation, we constructed a theoretical “phase map” that can assess both lamellar and nonlamellar membranes. We thoroughly validated this theory using well-known lipid mixtures under different phase-state conditions and enzymatically generated systems. Our results confirm that the use of MdSP is a powerful tool for obtaining quantitative information on both lamellar and nonlamellar structures. This study not only advances our understanding of the impact of lipid diversity on membrane organization but also provides a robust and general framework for the assessment of fluorescence properties that can be further extended to other probes. info:eu-repo/semantics/publishedVersion 2025-06-15 Fil: Socas, Luis B. P. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Socas, Luis B. P. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Valdivia-Pérez Jessica A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Valdivia-Pérez Jessica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Fanani María L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Fanani María L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. Fil: Ambroggio Ernesto E. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica, Ranwel Caputto, Argentina. Fil: Ambroggio Ernesto E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba, Argentina. 2024-06-18T18:20:11Z 2024-06-14 article Socas, L. B. P., Valdivia-Pérez, J. A., Fanani, M. L., & Ambroggio, E. E. (2024). Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? Journal of American Chemical Society. https://pubs.acs.org/doi/full/10.1021/jacs.4c03443 http://hdl.handle.net/11086/552327 1520-5126 https://pubs.acs.org/doi/full/10.1021/jacs.4c03443 spa Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ American Chemical Society |
| spellingShingle | Chemical structure Interfaces Lipids Membranes Phase transitions Socas, Luis B. P. Valdivia Pérez, Jessica A. Fanani, María L. Ambroggio, Ernesto E. Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title | Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title_full | Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title_fullStr | Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title_full_unstemmed | Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title_short | Multidimensional Spectral Phasors of LAURDAN’s Excitation–Emission Matrices: The Ultimate Sensor for Lipid Phases? |
| title_sort | multidimensional spectral phasors of laurdan s excitation emission matrices the ultimate sensor for lipid phases |
| topic | Chemical structure Interfaces Lipids Membranes Phase transitions |
| url | http://hdl.handle.net/11086/552327 https://pubs.acs.org/doi/full/10.1021/jacs.4c03443 |
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