AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation
Fil: Tessi, Tomás M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | conferenceObject |
| Language: | eng |
| Published: |
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/11086/551388 |
| _version_ | 1801216752014589952 |
|---|---|
| author | Tessi, Tomás M. Brumm, Sabine Winklbauer, Eva Lescano, Carlos I. Martini, Carolina Maurino, Veronica G. Grefen, Christopher Wanke, Dierk Klaus, Harter Desimone, Marcelo |
| author_facet | Tessi, Tomás M. Brumm, Sabine Winklbauer, Eva Lescano, Carlos I. Martini, Carolina Maurino, Veronica G. Grefen, Christopher Wanke, Dierk Klaus, Harter Desimone, Marcelo |
| author_sort | Tessi, Tomás M. |
| collection | Repositorio Digital Universitario |
| description | Fil: Tessi, Tomás M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. |
| format | conferenceObject |
| id | rdu-unc.551388 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| record_format | dspace |
| spelling | rdu-unc.5513882024-04-12T06:25:00Z AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation Tessi, Tomás M. Brumm, Sabine Winklbauer, Eva Lescano, Carlos I. Martini, Carolina Maurino, Veronica G. Grefen, Christopher Wanke, Dierk Klaus, Harter Desimone, Marcelo Arabidopsis Transporte Citocininas Azg1 Fil: Tessi, Tomás M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Brumm, Sabine. Centre for Molecular Plant Biology; Alemania. Fil: Winklbauer, Eva. Centre for Molecular Plant Biology; Alemania. Fil: Lescano, Carlos I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Martini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Maurino, Veronica G. University of Cologne; Alemania. Fil: Grefen, Christopher. Centre for Molecular Plant Biology; Alemania. Fil: Wanke, Dierk. Centre for Molecular Plant Biology; Alemania. Fil: Klaus, Harter. Centre for Molecular Plant Biology; Alemania. Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. AZG (AZa-Guanin resistant) is a gene family first described in fungi as purine transporters. Two members of this family, Azg1 and Azg2, are encoded in the Arabidopsis genome. Although they are able to transport purines when functionally expressed in yeast, their physiological role has not been addressed. In the present work, we found evidences suggesting that Azg2 is involved in lateral root development regulation. The function of Azg2 as a cytokinin (CK) transporter could be demonstrated in vivo. AZG2 is expressed in a limited number of cells surrounding the lateral root primordia (LRP). Azg2 promoter activity is present since stage one of LRPs and persist even when the lateral root is emerged. Moreover, initial approaches to AZG2 subcellular localization revealed that the transporter is localized at the plasma membrane. We also found that Azg2 expression is induced by auxins being the transcription factor ARF7 necessary for gene activation. Analysis of transgenic lines showed differences in root architecture. Azg2 KO lines showed more LR density than Wt plants, while overexpression lines (OEs) presented a conditional phenotype with lower LR density. Furthermore, when plants were treated with CKs, the differences between phenotypes increased significantly. KOs lines showed resistance to CKs, whereas an enhanced toxicity in the OEs was observed. All this data encourage us to propose Azg2 as a key factor in cytokinin inhibition of lateral root development. Fil: Wanke, Dierk. Centre for Molecular Plant Biology; Alemania. http://www.ipmb2015.org/Abstracts.php Fil: Tessi, Tomás M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Brumm, Sabine. Centre for Molecular Plant Biology; Alemania. Fil: Winklbauer, Eva. Centre for Molecular Plant Biology; Alemania. Fil: Lescano, Carlos I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Martini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Maurino, Veronica G. University of Cologne; Alemania. Fil: Grefen, Christopher. Centre for Molecular Plant Biology; Alemania. Fil: Wanke, Dierk. Centre for Molecular Plant Biology; Alemania. Fil: Klaus, Harter. Centre for Molecular Plant Biology; Alemania. Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Bioquímica y Biología Molecular (ídem 3.1.10) 2024-04-11T12:09:17Z 2024-04-11T12:09:17Z 2015 conferenceObject http://hdl.handle.net/11086/551388 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Electrónico y/o Digital |
| spellingShingle | Arabidopsis Transporte Citocininas Azg1 Tessi, Tomás M. Brumm, Sabine Winklbauer, Eva Lescano, Carlos I. Martini, Carolina Maurino, Veronica G. Grefen, Christopher Wanke, Dierk Klaus, Harter Desimone, Marcelo AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title | AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title_full | AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title_fullStr | AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title_full_unstemmed | AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title_short | AZG2 transporter plays a key role in auxin/cytokinin lateral root development regulation |
| title_sort | azg2 transporter plays a key role in auxin cytokinin lateral root development regulation |
| topic | Arabidopsis Transporte Citocininas Azg1 |
| url | http://hdl.handle.net/11086/551388 |
| work_keys_str_mv | AT tessitomasm azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT brummsabine azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT winklbauereva azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT lescanocarlosi azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT martinicarolina azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT maurinoveronicag azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT grefenchristopher azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT wankedierk azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT klausharter azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation AT desimonemarcelo azg2transporterplaysakeyroleinauxincytokininlateralrootdevelopmentregulation |