cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans
7 p.
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/publishedVersion |
| Language: | eng |
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American Society of Tropial Medicine and Hygiene
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11086/550125 |
| _version_ | 1801214240575455232 |
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| author | Grosso, Carla Gisela Stroppa, María Mercedes García, Beatríz Alicia Varela, Gonzalo Matías |
| author_facet | Grosso, Carla Gisela Stroppa, María Mercedes García, Beatríz Alicia Varela, Gonzalo Matías |
| author_sort | Grosso, Carla Gisela |
| collection | Repositorio Digital Universitario |
| description | 7 p. |
| format | info:eu-repo/semantics/publishedVersion |
| id | rdu-unc.550125 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2023 |
| publisher | American Society of Tropial Medicine and Hygiene |
| record_format | dspace |
| spelling | rdu-unc.5501252023-12-07T11:44:10Z cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans Grosso, Carla Gisela Stroppa, María Mercedes García, Beatríz Alicia Varela, Gonzalo Matías NADPH CYTOCHROME P450 REDUCTASE GENE TRIATOMA INFESTANS CHAGAS DISEASE VECTOR 7 p. info:eu-repo/semantics/publishedVersion Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Grosso, Carla Gisela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Stroppa, María Mercedes. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Varela, Gonzalo Matías. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Stroppa, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: García, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: García, Beatriz Alicia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Pyrethroid resistance has been detected in Triatoma infestans (Hemiptera: Reduviidae), which was atributed to target site insensitivity and increased oxidative metabolism of the insecticide by cytochrome P450s. NADPH cytochrome P450 reductase (CPR) plays an essential role in transferring electrons from NADPH to the P450-substrate complex. In this study, the full length CPR cDNA of T. infestans was isolated and gene expression was determined by quantitative PCR. The open reading frame is 2046 bp long, encoding a protein of 682 amino acids. Amino acid sequence analysis indicates that the T. infestans CPR and the putative Rhodnius prolixus and Triatoma dimidiata CPRs, present conserved ligand-binding domains. Congruently with a previous study of our laboratory, in which the expression of three cytochrome P450 genes (CYP4EM7, CYP3085B1, and CYP3092A6 genes) was induced by deltamethrin, the levels of T. infestans CPR mRNA were up-regulated in the fat body of fifth instar nymphs after topical application of deltamethrin. Besides, as it was observed in the CYP4EM7 gene, it was detected overexpression of the CPR gene in the most resistant strain of T. infestans included in the study. These results suggest that CPR plays an essential role in P450-mediated resistance of T. infestans to insecticides. info:eu-repo/semantics/publishedVersion Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Grosso, Carla Gisela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Stroppa, María Mercedes. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Varela, Gonzalo Matías. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Fil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Stroppa, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: García, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: García, Beatriz Alicia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bioquímica y Biología Molecular; Argentina. Bioquímica y Biología Molecular (ídem 3.1.10) 2023-12-06T18:30:51Z 2023-12-06T18:30:51Z 2018 article 0002-9637 http://hdl.handle.net/11086/550125 1476-1645 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso American Society of Tropial Medicine and Hygiene |
| spellingShingle | NADPH CYTOCHROME P450 REDUCTASE GENE TRIATOMA INFESTANS CHAGAS DISEASE VECTOR Grosso, Carla Gisela Stroppa, María Mercedes García, Beatríz Alicia Varela, Gonzalo Matías cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title | cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title_full | cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title_fullStr | cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title_full_unstemmed | cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title_short | cDNA isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome P450 reductase gene in the Chagas disease vector Triatoma infestans |
| title_sort | cdna isolation and expression of nicotinamide adenine dinucleotide phosphate dependent cytochrome p450 reductase gene in the chagas disease vector triatoma infestans |
| topic | NADPH CYTOCHROME P450 REDUCTASE GENE TRIATOMA INFESTANS CHAGAS DISEASE VECTOR |
| url | http://hdl.handle.net/11086/550125 |
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