Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina.
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| Format: | publishedVersion |
| Language: | eng |
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2022
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| Online Access: | http://hdl.handle.net/11086/28112 http://dx.doi.org/10.1021/jp410894x |
| _version_ | 1801213495264411648 |
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| author | Clop, Eduardo Matias Chattah, Ana Karina Perillo, María Angélica |
| author_facet | Clop, Eduardo Matias Chattah, Ana Karina Perillo, María Angélica |
| author_sort | Clop, Eduardo Matias |
| collection | Repositorio Digital Universitario |
| description | Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina. |
| format | publishedVersion |
| id | rdu-unc.28112 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2022 |
| record_format | dspace |
| spelling | rdu-unc.281122022-10-13T11:07:52Z Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s Clop, Eduardo Matias Chattah, Ana Karina Perillo, María Angélica Lipid vesicles Peg Pe-Peg Nuclear magnetic resonance NMR Water Dynamics publishedVersion Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina. Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Chattah, Ana Karina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Fil: Chattah, Ana Karina. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Fil: Chattah, Ana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Física Enrique Gaviola; Argentina. Fil: Perillo, María Angélica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina. Fil: Perillo, María Angélica. Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Perillo, María Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains. publishedVersion Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina. Fil: Clop, Eduardo Matias. Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Chattah, Ana Karina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Fil: Chattah, Ana Karina. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Fil: Chattah, Ana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Física Enrique Gaviola; Argentina. Fil: Perillo, María Angélica. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Química Biológica; Argentina. Fil: Perillo, María Angélica. Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Fil: Perillo, María Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Biofísica 2022-08-10T16:52:04Z 2014-05-08 article http://hdl.handle.net/11086/28112 http://dx.doi.org/10.1021/jp410894x http://dx.doi.org/10.1021/jp410894x eng Attribution-NonCommercial-NoDerivatives 4.0 International restrictedAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Impreso; Electrónico y/o Digital ISSN: 1520-6106 e-ISSN: 1520-5207 |
| spellingShingle | Lipid vesicles Peg Pe-Peg Nuclear magnetic resonance NMR Water Dynamics Clop, Eduardo Matias Chattah, Ana Karina Perillo, María Angélica Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title | Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title_full | Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title_fullStr | Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title_full_unstemmed | Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title_short | Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s |
| title_sort | water and membrane dynamics in suspensions of lipid vesicles functionalized with poly ethylene glycol s |
| topic | Lipid vesicles Peg Pe-Peg Nuclear magnetic resonance NMR Water Dynamics |
| url | http://hdl.handle.net/11086/28112 http://dx.doi.org/10.1021/jp410894x http://dx.doi.org/10.1021/jp410894x |
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