Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
1 p.
| Main Authors: | , |
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| Format: | conferenceObject |
| Language: | eng |
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International Union of Immunological Societies (IUIS)
2020
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| Online Access: | http://hdl.handle.net/11086/16452 |
| _version_ | 1801215035494629376 |
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| author | Novak, Ivón Orquera, Abel |
| author_facet | Novak, Ivón Orquera, Abel |
| author_sort | Novak, Ivón |
| collection | Repositorio Digital Universitario |
| description | 1 p. |
| format | conferenceObject |
| id | rdu-unc.16452 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2020 |
| publisher | International Union of Immunological Societies (IUIS) |
| record_format | dspace |
| spelling | rdu-unc.164522023-08-30T14:09:44Z Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease Novak, Ivón Orquera, Abel Antigen processing and presentation Ultrastucture Inmunological Synapses Macrophages Lymphocytes 1 p. Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. The processing and antigen presentation are involved in human macrophage lymphocyte rosette (MLR) and in this phenomenon of multiple immune synapse (IS) the autologous antigens of senescent neutrophils are presented by endocitic way like phagocytosis of cells undergoing apoptosis naturally in the body. In Chagas disease cardiomyopathy T. cruzi is the etiologic agent but also autoimmune phenomena have been described and observed an increment in occurrence of this phenomenon and CD4 PAS-positive lymphocytes producing IFN gamma are involved. Material and methods: healthy human blood samples, anticoagulated with heparin (n = 10) and chagasic samples (n=6) (Blood Bank, UNC) Autologous cultures in TC199 medium (SIGMA, St. Louis, MO). Samples: 48, 72, 96 and 120 h. MLR technique. PAS technique. Samples of MLRs underwent electron microscopy. Results: MLRs of chagasic patients involved more lymphocytes. Significant differences were observed between PAS positive lymphocytes from MLRs (p <0.01). In Chagas cultures were observed plasma cells and neutrophils up to 48 h. Ultrastructure: redistribution of mitochondria in lymphocytes of MLRs, to the IS area. We observed multivesicular, multilamellar and tubular structures in spatial organization of MIIC along time culture in macrophages. In lymphocytes were observed differences in arrangement of chromatin between healthy individuals and Chagas patients as in previous works. Is noted that in cytoplasm were observed double-membrane vesicles to a greater extent in Chagas patients, we postulate costimulator B7 molecules carriers and we are preparing further studies to immunostaining. In all cases observed exosomes, IS and MLR phenomenon greater degree in chagasic patients cultures. https://www.frontiersin.org/10.3389/conf.fimmu.2013.02.00875/event_abstract Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Inmunología 2020-10-07T17:47:10Z 2020-10-07T17:47:10Z 2013 conferenceObject 1664-3224 doi: 10.3389/conf.fimmu.2013.02.00875 http://hdl.handle.net/11086/16452 eng 11086/14404 Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ Electrónico y/o Digital International Union of Immunological Societies (IUIS) International Union of Immunological Societies (IUIS) Novak IT and Orquera AD (2013). Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00875 |
| spellingShingle | Antigen processing and presentation Ultrastucture Inmunological Synapses Macrophages Lymphocytes Novak, Ivón Orquera, Abel Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title | Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title_full | Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title_fullStr | Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title_full_unstemmed | Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title_short | Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease |
| title_sort | ultrastructure of human autologous macrophage lymphocyte rosettes in chagas disease |
| topic | Antigen processing and presentation Ultrastucture Inmunological Synapses Macrophages Lymphocytes |
| url | http://hdl.handle.net/11086/16452 |
| work_keys_str_mv | AT novakivon ultrastructureofhumanautologousmacrophagelymphocyterosettesinchagasdisease AT orqueraabel ultrastructureofhumanautologousmacrophagelymphocyterosettesinchagasdisease |