An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenas...
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| Format: | publishedVersion |
| Language: | eng |
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2019
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| Online Access: | http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 |
| _version_ | 1801214365933764608 |
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| author | Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
| author_facet | Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
| author_sort | Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
| collection | Repositorio Digital Universitario |
| description | Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts. |
| format | publishedVersion |
| id | rdu-unc.13456 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2019 |
| record_format | dspace |
| spelling | rdu-unc.134562022-06-09T13:01:17Z An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS publishedVersion Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts. publishedVersion Odontología, Medicina y Cirugía Oral 2019-10-29T16:05:41Z 2019-10-29T16:05:41Z 2013 article http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 eng Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso; Electrónico y/o Digital |
| spellingShingle | GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title_full | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title_fullStr | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title_full_unstemmed | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title_short | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
| title_sort | in vitro biocompatibility study of conventional and resin modified glass ionomer cements |
| topic | GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS |
| url | http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 |
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