Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium
Alfalfa breeding programs require environmentally friendly alternatives to improve plant growth and health. Plant Growth Promoting Rhizobacteria (PGPR) and endophytic bacteria offer a biological solution as they can inhibitphytopathogenic fungi by producing antifungal compounds or enzymes. In Argent...
| Main Authors: | , , , , , , , , |
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| Format: | Online |
| Language: | eng |
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Facultad de Ciencias Agropecuarias
2024
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/41895 |
| _version_ | 1806010984687468544 |
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| author | Moreno, María Valeria Arolfo, Valeria López, José Luis Erdozain, Sofía Agostina Bigatton, Ezequiel Darío Ayoub, Ibrahim Lucini, Enrique Iván Lagares, Antonio Odorizzi, Ariel |
| author_facet | Moreno, María Valeria Arolfo, Valeria López, José Luis Erdozain, Sofía Agostina Bigatton, Ezequiel Darío Ayoub, Ibrahim Lucini, Enrique Iván Lagares, Antonio Odorizzi, Ariel |
| author_sort | Moreno, María Valeria |
| collection | Portal de Revistas |
| description | Alfalfa breeding programs require environmentally friendly alternatives to improve plant growth and health. Plant Growth Promoting Rhizobacteria (PGPR) and endophytic bacteria offer a biological solution as they can inhibitphytopathogenic fungi by producing antifungal compounds or enzymes. In Argentina, alfalfa is primarily affected by Colletotrichum trifolii (Ct) and Phytophthora megasperma (Pm). The objectives of this study were to select bacterial isolates with antifungal inhibition against Ct and Pm and identify a biocontroller isolate compatible with the rhizobia INTA5 has and with low-N2O emissions, to constitute an eco-friendly bacterial consortium. Ten isolates demonstrated antifungal inhibition, with mean Inhibition Percentage (IP) values between 41.74-91.28 for Ct and 56.23-77.59 for Pm compared to the control. Among these isolates, B2, B4, SC6, and MN1 showed the highest inhibition performance and were selected to evaluate their compatibility with INTA5. Only B4 exhibited compatibility, which was further evaluated against Ct in an experiment with alfalfa seedlings under controlled conditions. The alfalfaseedlings treated with B4 increased the plant survival number against Ct. The availability of a bacterial consortium that promotes biocontrol and biological nitrogen fixation represents the preferred strategy for alfalfa breeding programs. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-41895 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2024 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-418952024-07-05T18:24:53Z Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium Selección de cepas bacterianas con capacidad antifúngica contra fitopatógenos de alfalfa para constituir un consorcio bacteriano Moreno, María Valeria Arolfo, Valeria López, José Luis Erdozain, Sofía Agostina Bigatton, Ezequiel Darío Ayoub, Ibrahim Lucini, Enrique Iván Lagares, Antonio Odorizzi, Ariel Medicago sativa L. control biológico mejoramiento endófitos rizobacterias promotoras del crecimiento vegetal (RPCV) Medicago sativa L. biological control breeding strategies endophytes plant growth promoting rhizobacteria (PGPR) Alfalfa breeding programs require environmentally friendly alternatives to improve plant growth and health. Plant Growth Promoting Rhizobacteria (PGPR) and endophytic bacteria offer a biological solution as they can inhibitphytopathogenic fungi by producing antifungal compounds or enzymes. In Argentina, alfalfa is primarily affected by Colletotrichum trifolii (Ct) and Phytophthora megasperma (Pm). The objectives of this study were to select bacterial isolates with antifungal inhibition against Ct and Pm and identify a biocontroller isolate compatible with the rhizobia INTA5 has and with low-N2O emissions, to constitute an eco-friendly bacterial consortium. Ten isolates demonstrated antifungal inhibition, with mean Inhibition Percentage (IP) values between 41.74-91.28 for Ct and 56.23-77.59 for Pm compared to the control. Among these isolates, B2, B4, SC6, and MN1 showed the highest inhibition performance and were selected to evaluate their compatibility with INTA5. Only B4 exhibited compatibility, which was further evaluated against Ct in an experiment with alfalfa seedlings under controlled conditions. The alfalfaseedlings treated with B4 increased the plant survival number against Ct. The availability of a bacterial consortium that promotes biocontrol and biological nitrogen fixation represents the preferred strategy for alfalfa breeding programs. El programa de mejoramiento de alfalfa requiere alternativas más amigables con el ambiente para incrementar la sanidad del cultivo. Las rizobacterias promotoras del crecimiento vegetal (RPCV) y endófitos de semillas constituyen una opción para inhibir hongos patógenos por la producción de compuestos antifúngicos o enzimas hidrolíticas. En Argentina, la alfalfa es afectada principalmente por Colletotrichum trifolii (Ct) y Phytophthora megasperma (Pm). Los objetivos fueron seleccionar bacterias con capacidad antifúngica contra Ct y Pm, e identificar una compatible con el rizobio INTA5 con bajaemisión de N2O para constituir un consorcio bacteriano sustentable. Diez cepas mostraron capacidad antifúngica con medias de porcentaje de inhibición (PI) entre 41,74-91,28 para Ct y 56,23-77,59 relativos al control para Pm. B2, B4, SC6 y MN1 fueron seleccionadas por su mayor capacidad de biocontrol y se estudió la compatibilidad con INTA5. Sólo B4 resultó compatible, por ello fue evaluada contra Ct sobre plántulas de alfalfa en experimentos bajo condiciones controladas. Esta cepa incrementó el número de plántulas sobrevivientes cuando las mismas fueron inoculadas con Ct. La disponibilidad de un consorcio bacteriano que estimule tanto el biocontrol como la fijación biológica de nitrógeno representa la estrategia preferida por el programa de mejoramiento de alfalfa. Facultad de Ciencias Agropecuarias 2024-07-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/41895 AgriScientia; Vol. 41 No. 1 (2024); 73-82 AgriScientia; Vol. 41 Núm. 1 (2024); 73-82 1668-298X 10.31047/1668.298x.v41.n1 eng https://revistas.unc.edu.ar/index.php/agris/article/view/41895/45696 Derechos de autor 2024 María Valeria Moreno, Valeria Arolfo, José Luis López, So´fía Agostina Erdozain, Ezequiel Darío Bigatton, Ibrahim Ayoub, Enrique Iván Lucini, Antonio Lagares, Ariel Odorizzi https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | Medicago sativa L. control biológico mejoramiento endófitos rizobacterias promotoras del crecimiento vegetal (RPCV) Medicago sativa L. biological control breeding strategies endophytes plant growth promoting rhizobacteria (PGPR) Moreno, María Valeria Arolfo, Valeria López, José Luis Erdozain, Sofía Agostina Bigatton, Ezequiel Darío Ayoub, Ibrahim Lucini, Enrique Iván Lagares, Antonio Odorizzi, Ariel Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title | Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title_alt | Selección de cepas bacterianas con capacidad antifúngica contra fitopatógenos de alfalfa para constituir un consorcio bacteriano |
| title_full | Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title_fullStr | Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title_full_unstemmed | Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title_short | Selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| title_sort | selection of bacterial isolates with fungal inhibition against alfalfa phytopathogens to constitute a bacterial consortium |
| topic | Medicago sativa L. control biológico mejoramiento endófitos rizobacterias promotoras del crecimiento vegetal (RPCV) Medicago sativa L. biological control breeding strategies endophytes plant growth promoting rhizobacteria (PGPR) |
| topic_facet | Medicago sativa L. control biológico mejoramiento endófitos rizobacterias promotoras del crecimiento vegetal (RPCV) Medicago sativa L. biological control breeding strategies endophytes plant growth promoting rhizobacteria (PGPR) |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/41895 |
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