(2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor
Hibiscus cannabinus L. is an annual species cultivated for its fibers. The morphological description of the seeds of the cultivars ‘Endora’ and ‘Tainung' 1’ was broadened, a tetrazolium test procedure was adapted and adjusted, and the embryos were classified according to their viability and vig...
| Main Authors: | , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Facultad de Ciencias Agropecuarias
2024
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| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/40982 |
| _version_ | 1808111812986535936 |
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| author | Bornand, Alejandra del Valle Agüero, César Germán Molinelli, María Laura |
| author_facet | Bornand, Alejandra del Valle Agüero, César Germán Molinelli, María Laura |
| author_sort | Bornand, Alejandra del Valle |
| collection | Portal de Revistas |
| description | Hibiscus cannabinus L. is an annual species cultivated for its fibers. The morphological description of the seeds of the cultivars ‘Endora’ and ‘Tainung' 1’ was broadened, a tetrazolium test procedure was adapted and adjusted, and the embryos were classified according to their viability and vigor. Cells from the seed coat layers participate in the seed coloration and ornamentation. The imbibition cap and the isthmus were identified, and their function wasanalyzed. Lipids and proteins were recognized in the mesophyll of cotyledons and the absence of areoles in the epidermis. Using the tetrazolium test, staining patterns were established to classify the seeds according to their viability. Both cultivars presented acceptable viability values. The germination test supported the proposed methodology for tetrazolium. Novel contributions are made to seed morphology, and structure-function relationships for water intake are proposed. The seeds of both cultivars share the morphological characteristics that are fundamental to the establishment of evaluation criteria in the tetrazolium biochemical test. The adapted tetrazolium viability test protocol allowed the seeds classification as either viable into three categories (high, medium, and low vigor) and non-viable seeds. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-40982 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2024 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-409822024-07-05T18:25:02Z (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor Aportes a la morfología de semillas de Hibiscus cannabinus L. y ajuste de la prueba de tetrazolio para estimar viabilidad y vigor Bornand, Alejandra del Valle Agüero, César Germán Molinelli, María Laura germination kenaf Malvaceae quality seed morphology calidad germinación kenaf Malvaceae morfología de semilla Hibiscus cannabinus L. is an annual species cultivated for its fibers. The morphological description of the seeds of the cultivars ‘Endora’ and ‘Tainung' 1’ was broadened, a tetrazolium test procedure was adapted and adjusted, and the embryos were classified according to their viability and vigor. Cells from the seed coat layers participate in the seed coloration and ornamentation. The imbibition cap and the isthmus were identified, and their function wasanalyzed. Lipids and proteins were recognized in the mesophyll of cotyledons and the absence of areoles in the epidermis. Using the tetrazolium test, staining patterns were established to classify the seeds according to their viability. Both cultivars presented acceptable viability values. The germination test supported the proposed methodology for tetrazolium. Novel contributions are made to seed morphology, and structure-function relationships for water intake are proposed. The seeds of both cultivars share the morphological characteristics that are fundamental to the establishment of evaluation criteria in the tetrazolium biochemical test. The adapted tetrazolium viability test protocol allowed the seeds classification as either viable into three categories (high, medium, and low vigor) and non-viable seeds. Hibiscus cannabinus L. es una especie anual cultivada por sus fibras. Se profundizó la descripción morfológica de las semillas de los cultivares ‘Endora’ y ‘Tainung 1’, se adecuó y ajustó un procedimiento de prueba de tetrazolio y se clasificaron los embriones según su viabilidad y vigor. En la coloración y ornamentación de las semillas participan células de las capas del episperma. Se identificaron la tapa de imbibición y el istmo y se analizó su función. Se reconocieron lípidos y proteínas en el mesofilo de los cotiledones y la ausencia de aréolas en la epidermis. Mediante la prueba de tetrazolio se establecieron patrones de tinción para clasificar las semillas según su viabilidad. Ambos cultivares presentaron valores de viabilidad aceptables. La prueba de germinación respaldó la metodología propuesta para tetrazolio. Se realizan aportes novedosos sobre la morfología de las semillas y se proponen relaciones estructura-función para el ingreso de agua. Las semillas de ambos cultivares comparten características morfológicas fundamentales para establecer los criterios de evaluación en la prueba bioquímica de tetrazolio. La adaptación del protocolo de la prueba de viabilidad por tetrazolio permitió la clasificación de las semillas como viables en tres categorías (vigor alto, medio y bajo) y no viables. Facultad de Ciencias Agropecuarias 2024-07-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/40982 AgriScientia; Vol. 41 No. 1 (2024); 53-71 AgriScientia; Vol. 41 Núm. 1 (2024); 53-71 1668-298X 10.31047/1668.298x.v41.n1 spa https://revistas.unc.edu.ar/index.php/agris/article/view/40982/45697 Derechos de autor 2024 Alejandra del Valle Bornand, César Germán Agüero, María Laura Molinelli https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | germination kenaf Malvaceae quality seed morphology calidad germinación kenaf Malvaceae morfología de semilla Bornand, Alejandra del Valle Agüero, César Germán Molinelli, María Laura (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title | (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title_alt | Aportes a la morfología de semillas de Hibiscus cannabinus L. y ajuste de la prueba de tetrazolio para estimar viabilidad y vigor |
| title_full | (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title_fullStr | (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title_full_unstemmed | (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title_short | (2024). Contri- butions to the morphology of Hibiscus cannabinus L. seeds and ad- justment of the tetrazolium test to estimate viability and vigor |
| title_sort | 2024 contri butions to the morphology of hibiscus cannabinus l seeds and ad justment of the tetrazolium test to estimate viability and vigor |
| topic | germination kenaf Malvaceae quality seed morphology calidad germinación kenaf Malvaceae morfología de semilla |
| topic_facet | germination kenaf Malvaceae quality seed morphology calidad germinación kenaf Malvaceae morfología de semilla |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/40982 |
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