Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture
Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount of generated polymorphi...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | eng |
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Facultad de Ciencias Agropecuarias
2021
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/33260 |
| _version_ | 1811172645256822784 |
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| author | Ermini, J. L. Tenaglia, G. C. Parisod, C. Pratta, Guillermo R. Pratta, Guillermo R. |
| author_facet | Ermini, J. L. Tenaglia, G. C. Parisod, C. Pratta, Guillermo R. Pratta, Guillermo R. |
| author_sort | Ermini, J. L. |
| collection | Portal de Revistas |
| description | Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount of generated polymorphism, are necessary. In this work, preliminary results of somaclonal variation during early cycles of banana in vitro culture are reported. Four randomly selected regenerated plants from the fifth cycle and two samples from the mother plant were analyzed. A total of 36 AFLP primer combinations were assayed, and 24 of them produced amplicons varying among 50- 500 bp. The mother plant presented a total of 125 different amplicons while the regenerated plants jointly showed 131 different amplicons with a mean of 119.75 ± 3.97 per individual. High level of DNA polymorphism (24.43 %) was found among micropropagated plants and, additionally, the occurrence of somaclonal variation at earlier cycles was suggested by multivariate analysis of Principal Coordinates. In this study, somaclonal variation at early cycles of banana micropropagation was validated and the adequacy of AFLP technique to assess it at the molecular level was verified. The phenotypic effects of the detected somaclonal variations remain to be evaluated.
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| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-33260 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2021 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-332602022-03-22T16:59:54Z Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro Ermini, J. L. Tenaglia, G. C. Parisod, C. Pratta, Guillermo R. Pratta, Guillermo R. micropropagation Musa spp. genetic variability molecular plant breeding Principal Coordinates Analysis micropropagación Musa spp. variabilidad genética mejoramiento vegetal molecular Análisis de Coordenadas Principales Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount of generated polymorphism, are necessary. In this work, preliminary results of somaclonal variation during early cycles of banana in vitro culture are reported. Four randomly selected regenerated plants from the fifth cycle and two samples from the mother plant were analyzed. A total of 36 AFLP primer combinations were assayed, and 24 of them produced amplicons varying among 50- 500 bp. The mother plant presented a total of 125 different amplicons while the regenerated plants jointly showed 131 different amplicons with a mean of 119.75 ± 3.97 per individual. High level of DNA polymorphism (24.43 %) was found among micropropagated plants and, additionally, the occurrence of somaclonal variation at earlier cycles was suggested by multivariate analysis of Principal Coordinates. In this study, somaclonal variation at early cycles of banana micropropagation was validated and the adequacy of AFLP technique to assess it at the molecular level was verified. The phenotypic effects of the detected somaclonal variations remain to be evaluated. La micropropagación de banana para obtener plantas libres de virus frecuentemente provoca variación somaclonal que puede incrementar la variabilidad genética en cultivos de reproducción asexual. Es necesario explorar el ciclo del cultivo in vitro en que se produce esta variación así como cuantificar el porcentaje de polimorfismo. Este trabajo presenta resultados de variación somaclonal en ciclos tempranos de micropropagación de banana. Cuatro plantas tomadas al azar del quinto ciclo de regeneración y dos muestras de la planta madre se caracterizaron con 36 combinaciones de cebadores de AFLP. Veinticuatro combinaciones produjeron amplicones en un rango entre 50-500 pb. La planta madre presentó en total 125 amplicones mientras que en conjunto las plantas regeneradas mostraron 131 amplicones, con una media de 119,75 ± 3,97 por individuo. Se detectó un alto porcentaje de polimorfismo (24,43 %) en las plantas micropropagadas y, adicionalmente, análisis multivariados de coordenadas principales sugirieron la ocurrencia de variación somaclonal en ciclos de regeneración anteriores. En este estudio se validó la ocurrencia de variación somacloanl en ciclos tempranos de la micropropagación en banana y se verificó que la técnica de AFLP es adecuada para evaluarla a nivel molecular. Queda pendiente evaluar los efectos fenotípicos de estas variantes somaclonales. Facultad de Ciencias Agropecuarias 2021-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/agris/article/view/33260 10.31047/1668.298x.v38.n2.33260 AgriScientia; Vol. 38 No. 2 (2021); 143-148 AgriScientia; Vol. 38 Núm. 2 (2021); 143-148 1668-298X 10.31047/1668.298x.v38.n2 eng https://revistas.unc.edu.ar/index.php/agris/article/view/33260/36798 https://revistas.unc.edu.ar/index.php/agris/article/view/33260/37374 Derechos de autor 2021 J. L. Ermini, G. C. Tenaglia, C. Parisod, Guillermo R. Pratta https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | micropropagation Musa spp. genetic variability molecular plant breeding Principal Coordinates Analysis micropropagación Musa spp. variabilidad genética mejoramiento vegetal molecular Análisis de Coordenadas Principales Ermini, J. L. Tenaglia, G. C. Parisod, C. Pratta, Guillermo R. Pratta, Guillermo R. Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title_alt | Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_full | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title_fullStr | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title_full_unstemmed | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title_short | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture |
| title_sort | banana somaclonal variation assessed by amplified fragment length polymorphism profiles at early cycles of in vitro culture |
| topic | micropropagation Musa spp. genetic variability molecular plant breeding Principal Coordinates Analysis micropropagación Musa spp. variabilidad genética mejoramiento vegetal molecular Análisis de Coordenadas Principales |
| topic_facet | micropropagation Musa spp. genetic variability molecular plant breeding Principal Coordinates Analysis micropropagación Musa spp. variabilidad genética mejoramiento vegetal molecular Análisis de Coordenadas Principales |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/33260 |
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