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Germination, hydrotime and isotopic analysis of Vicia villosa Roth. under water and salinity stress
Vicia villosa is a potential forage resource in marginal areas associated with its natural reseeding and agronomic attributes. The objectives of this work were to record and analyze the water absorption pattern, determine the germination percentage, estimate the hydrotime parameters and establish th...
Vicia villosa is a potential forage resource in marginal areas associated with its natural reseeding and agronomic attributes. The objectives of this work were to record and analyze the water absorption pattern, determine the germination percentage, estimate the hydrotime parameters and establish the 13C y 15N isotopic composition of the seedling, when Vicia villosa germinates in water and salinity stress conditions. The assays to determine the water absorption pattern and the response to germination were carried out according to the treatment: control (distilled water) and the solutions with potential water (Ya): -0.3; -0.6; -0.8; -1 y -1.2 MPa. The osmolytes were poliethyleneglicol (PEG) and sodium chloride (NaCl). The isotopic composition of the total organic matter (MOT) was determined under control conditions, -0.8 and -1.0 MPa. The absorption of 125 % of water triggered germination. It was estimated that 90 % of germination would be achieved with Ya of -0.6 and -0.8 MPa in water and salinity stress. PEG and NaCl treatments showed an enrichment in 13C and a depletion in 15N with respect to the control. The experimental results lead to the conclusion that this species is more tolerant to salinity than to water stress.