Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR
Olive growing is affected by a syndrome that causes wilt in the tree top. One of the main microorganisms accountable for this disease is the fungus Verticillium dahliae, which causes verticillium wilt (VW). Two pathotypes are distinguished within V. dahliae, defoliating (D) and non-defoliating (ND),...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | spa |
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Facultad de Ciencias Agropecuarias
2021
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/28985 |
| _version_ | 1811172639887065088 |
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| author | Rattalino, Donna Otero, María Laura Moriconi , D. N. Rivera, P. C. |
| author_facet | Rattalino, Donna Otero, María Laura Moriconi , D. N. Rivera, P. C. |
| author_sort | Rattalino, Donna |
| collection | Portal de Revistas |
| description | Olive growing is affected by a syndrome that causes wilt in the tree top. One of the main microorganisms accountable for this disease is the fungus Verticillium dahliae, which causes verticillium wilt (VW). Two pathotypes are distinguished within V. dahliae, defoliating (D) and non-defoliating (ND), depending on symptom severity. The pathogen is generally diagnosed by polymerase chain reaction (PCR). Our objective was to compare the efficiency of two sets of primers in detecting the ND pathotype in olive plants, the only pathotype found in Argentina, by nested PCR. For this purpose, the commonly used primer combination NDf/NDr - INTND2f/INTND2r (PCR-I) and a new combination, INTNDf/INTNDr - INTND2f/INTND2r (PCR-II), were evaluated. Samples of symptomatic olive trees from 39 commercial fields from La Rioja province were analyzed. PCR-II had higher detection efficiency than PCR-I (57.8 % and 16.7 %, respectively). PCR-II amplified in almost all the olive cultivars, plant ages and VW disease severity levels, whereas PCR-I detected the fungus only in very susceptible cultivars and in trees with low VW severity levels. The use of PCR-II for the detection of V. dahliae in olive plants will provide more accurate incidence results and will contribute to a proper management of VW. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-28985 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2021 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-289852024-06-18T22:25:38Z Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR Mejora de la detección del patotipo no defoliante de Verticillium dahliae en olivo mediante PCR anidada Rattalino, Donna Otero, María Laura Moriconi , D. N. Rivera, P. C. verticilosis diagnóstico molecular Olea europae verticillium wilt molecular diagnosis Olea europae Olive growing is affected by a syndrome that causes wilt in the tree top. One of the main microorganisms accountable for this disease is the fungus Verticillium dahliae, which causes verticillium wilt (VW). Two pathotypes are distinguished within V. dahliae, defoliating (D) and non-defoliating (ND), depending on symptom severity. The pathogen is generally diagnosed by polymerase chain reaction (PCR). Our objective was to compare the efficiency of two sets of primers in detecting the ND pathotype in olive plants, the only pathotype found in Argentina, by nested PCR. For this purpose, the commonly used primer combination NDf/NDr - INTND2f/INTND2r (PCR-I) and a new combination, INTNDf/INTNDr - INTND2f/INTND2r (PCR-II), were evaluated. Samples of symptomatic olive trees from 39 commercial fields from La Rioja province were analyzed. PCR-II had higher detection efficiency than PCR-I (57.8 % and 16.7 %, respectively). PCR-II amplified in almost all the olive cultivars, plant ages and VW disease severity levels, whereas PCR-I detected the fungus only in very susceptible cultivars and in trees with low VW severity levels. The use of PCR-II for the detection of V. dahliae in olive plants will provide more accurate incidence results and will contribute to a proper management of VW. La olivicultura está afectada por un síndrome que genera marchitez en la copa del árbol. Uno de los microorganismos responsables es el hongo Verticillium dahliae, que provoca la enfermedad denominada verticilosis del olivo (VO). Según la severidad de los síntomas, se distinguen dos patotipos, defoliante (D) y no defoliante (ND). Su diagnóstico emplea la reacción en cadena de la polimerasa (PCR). Nuestro objetivo fue comparar la eficiencia de dos juegos de cebadores para detectar en planta el patotipo ND, el único presente en Argentina, mediante PCR anidada. Para ello se utilizaron los juegos de cebadores NDf/NDr - INTND2f/INTND2r (PCR-I), empleados comúnmente, y una nueva combinación, INTNDf/INTNDr - INTND2f/INTND2r (PCR-II). Se analizaron muestras de olivos sintomáticos de 39 fincas de la provincia deLa Rioja. PCR-II tuvo mayor eficiencia en la detección, 57,8 % frente a 16,7 % de PCR-I. PCR-II amplificó en casi todas las variedades, edades de plantas y niveles de severidad de la enfermedad, mientras que PCR-I detectó el hongo sólo en variedades muy susceptibles y con niveles de severidad leve. El uso de PCR-II para detectar a V. dahliae en olivo permitirá estudios más precisos sobre su incidencia que contribuirán al manejo adecuado de la verticilosis. Facultad de Ciencias Agropecuarias 2021-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text/html application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/28985 10.31047/1668.298x.v38.n1.28985 AgriScientia; Vol. 38 No. 1 (2021): ; 79-91 AgriScientia; Vol. 38 Núm. 1 (2021): ; 79-91 1668-298X 10.31047/1668.298x.v38.n1 spa https://revistas.unc.edu.ar/index.php/agris/article/view/28985/34965 https://revistas.unc.edu.ar/index.php/agris/article/view/28985/34010 Derechos de autor 2021 Donna Rattalino, María Laura Otero, D. N. Moriconi , P. C. Rivera https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | verticilosis diagnóstico molecular Olea europae verticillium wilt molecular diagnosis Olea europae Rattalino, Donna Otero, María Laura Moriconi , D. N. Rivera, P. C. Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title | Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title_alt | Mejora de la detección del patotipo no defoliante de Verticillium dahliae en olivo mediante PCR anidada |
| title_full | Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title_fullStr | Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title_full_unstemmed | Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title_short | Improved detection of the non-defoliant pathotype of Verticillium dahliae in olive trees using nested PCR |
| title_sort | improved detection of the non defoliant pathotype of verticillium dahliae in olive trees using nested pcr |
| topic | verticilosis diagnóstico molecular Olea europae verticillium wilt molecular diagnosis Olea europae |
| topic_facet | verticilosis diagnóstico molecular Olea europae verticillium wilt molecular diagnosis Olea europae |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/28985 |
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