Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production
Orchids (family Orchidaceae) are susceptible to several biotic and abiotic factors that affect their development and quality. Among them, Cymbidium mosaic virus (CymMV) and Odontoglossum ingspot virus (ORSV) are the most frequent pathogens, with the greatest economic impact worldwide. The mechanica...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | spa |
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Facultad de Ciencias Agropecuarias
2020
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| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/28268 |
| _version_ | 1811172639191859200 |
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| author | Dottori, C. A. de Breuil, S. Tuma, M. A. Nome, Claudia |
| author_facet | Dottori, C. A. de Breuil, S. Tuma, M. A. Nome, Claudia |
| author_sort | Dottori, C. A. |
| collection | Portal de Revistas |
| description | Orchids (family Orchidaceae) are susceptible to several biotic and abiotic factors that affect their development and quality. Among them, Cymbidium mosaic virus (CymMV) and Odontoglossum ingspot virus (ORSV) are the most frequent pathogens, with the greatest economic impact worldwide. The mechanical transmission of these viruses in addition to the predominantly agamic multiplication of orchids, make it necessary to work with virus-free plant material. The objective of this work was to develop diagnostic reagents for the detection of CymMV and ORSV. A polyclonal antiserum against CymMV + ORSV was made with a titer of 1:10000 determined by NC-ELISA. The antiserum was processed generating IgG and IgG-conjugated” anti CymMV+ORSV were obtained for use in DAS-ELISA in a dilution of 1:500. The immunodiagnosticcapacity of this technique was evaluated by analyzing samples from orchids with characteristic viral symptoms, collected from commercial nurseries in the city of Córdoba, Argentina. Viral infections were detected in 22 % of the samples analyzed and in 75 % of the nurseries surveyed. The ability of these reagents to identify CymMV, ORSV and mixed infections in orchids, will speed up analyzes and reduce their costs. This would represent an advantage for large-scale detections. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-28268 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2020 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-282682023-04-13T17:32:17Z Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production Producción de un antisuero policlonal para la detección de Cymbidium mosaic virus y Odontoglossum ringspot virus en orquídeas Dottori, C. A. de Breuil, S. Tuma, M. A. Nome, Claudia orquídeas fitovirología diagnóstico antisuero orchids phytovirology diagnosis antiserum Orchids (family Orchidaceae) are susceptible to several biotic and abiotic factors that affect their development and quality. Among them, Cymbidium mosaic virus (CymMV) and Odontoglossum ingspot virus (ORSV) are the most frequent pathogens, with the greatest economic impact worldwide. The mechanical transmission of these viruses in addition to the predominantly agamic multiplication of orchids, make it necessary to work with virus-free plant material. The objective of this work was to develop diagnostic reagents for the detection of CymMV and ORSV. A polyclonal antiserum against CymMV + ORSV was made with a titer of 1:10000 determined by NC-ELISA. The antiserum was processed generating IgG and IgG-conjugated” anti CymMV+ORSV were obtained for use in DAS-ELISA in a dilution of 1:500. The immunodiagnosticcapacity of this technique was evaluated by analyzing samples from orchids with characteristic viral symptoms, collected from commercial nurseries in the city of Córdoba, Argentina. Viral infections were detected in 22 % of the samples analyzed and in 75 % of the nurseries surveyed. The ability of these reagents to identify CymMV, ORSV and mixed infections in orchids, will speed up analyzes and reduce their costs. This would represent an advantage for large-scale detections. Las orquídeas (familia Orchidaceae) son susceptibles a diversos factores bióticos y abióticos que afectan su desarrollo y calidad. Entre ellos, Cymbidium mosaic virus (CymMV) y Odontoglossum ringspot virus (ORSV) son los patógenos más frecuentes y de mayor importancia económica a nivel mundial. La transmisión mecánica de estos virus y la multiplicación principalmente agámica de las orquídeas hacen necesario contar con material madre libre de virus. El objetivo de este trabajo fue elaborar reactivos de diagnóstico para la detección de CymMV y ORSV. Se elaboró un antisuero policlonal contra CymMV+ORSV que se tituló 1:10000 mediante NC-ELISA. El antisuero fue procesado y se obtuvieron IgG e IgG-conjugada anti CymMV+ORSV dilución1:500 para su utilización en DAS-ELISA. La capacidad inmunodiagnóstica de esta técnica se evaluó mediante el análisis de muestras provenientes de orquídeas con síntomas característicos de infección viral, recolectadas en viveros comerciales de la ciudad de Córdoba, Argentina. La presencia de virosis se detectó en el 22 % de las muestras estudiadas y en el 75 % de los establecimientos relevados. La capacidad de estos reactivos para identificar CymMV, ORSV e infecciones mixtas en orquídeas permitirá agilizar y economizar los análisis. Esto representaría una ventaja para las detecciones a gran escala. Facultad de Ciencias Agropecuarias 2020-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/28268 10.31047/1668.298x.v37.n2.28268 AgriScientia; Vol. 37 No. 2 (2020); 45-51 AgriScientia; Vol. 37 Núm. 2 (2020); 45-51 1668-298X 10.31047/1668.298x.v37.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/28268/41045 https://revistas.unc.edu.ar/index.php/agris/article/view/28268/32705 Derechos de autor 2020 C. A. Dottori, S. de Breuil, M. A. Tuma, Claudia Nome https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | orquídeas fitovirología diagnóstico antisuero orchids phytovirology diagnosis antiserum Dottori, C. A. de Breuil, S. Tuma, M. A. Nome, Claudia Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title | Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title_alt | Producción de un antisuero policlonal para la detección de Cymbidium mosaic virus y Odontoglossum ringspot virus en orquídeas |
| title_full | Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title_fullStr | Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title_full_unstemmed | Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title_short | Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production |
| title_sort | polyclonal antiserum for the detection of cymbidium mosaic virus and odontoglossum ringspot virus production |
| topic | orquídeas fitovirología diagnóstico antisuero orchids phytovirology diagnosis antiserum |
| topic_facet | orquídeas fitovirología diagnóstico antisuero orchids phytovirology diagnosis antiserum |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/28268 |
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