Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton
Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb. is a disease that affects olive (Olea europaea L.) and cotton crops, among others. According to their pathogenicity, V. dahliae isolates can be classified as defoliating (D) and non-defoliating (ND). The objectives of this...
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Facultad de Ciencias Agropecuarias
2021
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/26937 |
| _version_ | 1811172636772794368 |
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| author | Gonzalez, Valeria Paccioretti, M. Rattalino, D. Taborda, R. J. Carrasco, F. Arias, F. Otero, M. L. |
| author_facet | Gonzalez, Valeria Paccioretti, M. Rattalino, D. Taborda, R. J. Carrasco, F. Arias, F. Otero, M. L. |
| author_sort | Gonzalez, Valeria |
| collection | Portal de Revistas |
| description | Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb. is a disease that affects olive (Olea europaea L.) and cotton crops, among others. According to their pathogenicity, V. dahliae isolates can be classified as defoliating (D) and non-defoliating (ND). The objectives of this study were: i) to determine the pathotypes of a collection of 42 V. dahliae isolates from olive plants in the provinces of Catamarca, Córdoba, La Rioja and Mendoza kept at IPAVE-INTA Córdoba, and (ii) to evaluate the virulence of eight recently isolated isolates belonging to this collection. Nested PCR was used for molecular analysis of the 42 fungal isolates and virulence evaluation was performed by artificial inoculations on cotton seedlings with a completely randomized design. The total of the isolates was characterized as ND. Two strains from La Rioja, two from Catamarca and one from Mendoza, were significantly more virulent (p< 0.05) for the relative area under disease progress curve, final mean severity, average severity percentage and severity index. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-26937 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2021 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-269372024-06-18T22:25:38Z Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton Determinación de patotipos de Verticillium dahliae Kleb. provenientes de las regiones olivícolas del centro y oeste de Argentina y pruebas de virulencia en algodón Gonzalez, Valeria Paccioretti, M. Rattalino, D. Taborda, R. J. Carrasco, F. Arias, F. Otero, M. L. verticillium wilt pathotype isolates severity olive verticilosis patotipo aislamientos severidad olivo Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb. is a disease that affects olive (Olea europaea L.) and cotton crops, among others. According to their pathogenicity, V. dahliae isolates can be classified as defoliating (D) and non-defoliating (ND). The objectives of this study were: i) to determine the pathotypes of a collection of 42 V. dahliae isolates from olive plants in the provinces of Catamarca, Córdoba, La Rioja and Mendoza kept at IPAVE-INTA Córdoba, and (ii) to evaluate the virulence of eight recently isolated isolates belonging to this collection. Nested PCR was used for molecular analysis of the 42 fungal isolates and virulence evaluation was performed by artificial inoculations on cotton seedlings with a completely randomized design. The total of the isolates was characterized as ND. Two strains from La Rioja, two from Catamarca and one from Mendoza, were significantly more virulent (p< 0.05) for the relative area under disease progress curve, final mean severity, average severity percentage and severity index. La verticilosis causada por el hongo habitante de suelo Verticillium dahliae Kleb. es una enfermedad que afecta el cultivo del olivo (Olea europaea L.) y algodón (Gossypium hirsutum L.), entre otros. De acuerdo a su patogenicidad, los aislados del patógeno pueden clasificarse como patotipos defoliantes (D) y no defoliantes (ND). Los objetivos de este estudio fueron: i) determinar los patotipos de una colección de 42 aislados de V. dahliae provenientes de plantas de olivo de las provincias de Catamarca, Córdoba, La Rioja y Mendoza existente en el IPAVE-INTA Córdoba, y ii) evaluar la virulencia de ocho cepas recientemente aisladas pertenecientes a dicha colección. Se utilizó la técnica de PCR anidado para el análisis molecular de los aislados del hongo y la evaluación de la virulencia se realizó mediante inoculaciones artificiales en plantines de algodón con un diseño completamente aleatorizado. El 100 % de los aislados fueron caracterizados como ND. Dos cepas provenientes de La Rioja, dos de Catamarca y una de Mendoza, fueron significativamente más virulentas (p< 0,05) para el área bajo la curva de progreso de la enfermedad relativa, severidad media final, porcentaje de severidad media e índice de severidad. Facultad de Ciencias Agropecuarias 2021-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text/html application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/26937 10.31047/1668.298x.v38.n1.26937 AgriScientia; Vol. 38 No. 1 (2021): ; 59-69 AgriScientia; Vol. 38 Núm. 1 (2021): ; 59-69 1668-298X 10.31047/1668.298x.v38.n1 spa https://revistas.unc.edu.ar/index.php/agris/article/view/26937/34963 https://revistas.unc.edu.ar/index.php/agris/article/view/26937/33999 Derechos de autor 2021 Valeria Gonzalez, M. Paccioretti, D. Rattalino, R. J. Taborda, F. Carrasco, F. Arias, M. L. Otero https://creativecommons.org/licenses/by-sa/4.0 |
| spellingShingle | verticillium wilt pathotype isolates severity olive verticilosis patotipo aislamientos severidad olivo Gonzalez, Valeria Paccioretti, M. Rattalino, D. Taborda, R. J. Carrasco, F. Arias, F. Otero, M. L. Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title | Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title_alt | Determinación de patotipos de Verticillium dahliae Kleb. provenientes de las regiones olivícolas del centro y oeste de Argentina y pruebas de virulencia en algodón |
| title_full | Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title_fullStr | Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title_full_unstemmed | Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title_short | Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton |
| title_sort | determination of verticillium dahliae kleb pathotypes from the olive regions of central and western argentina and virulence tests in cotton |
| topic | verticillium wilt pathotype isolates severity olive verticilosis patotipo aislamientos severidad olivo |
| topic_facet | verticillium wilt pathotype isolates severity olive verticilosis patotipo aislamientos severidad olivo |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/26937 |
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