Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds
The objective of the study was to establish guidelines for the viability test of seeds by tetrazolium in Cenchrus ciliaris using the Panicum protocols (ISTA, 2012). We worked with fascicles (dispersion unit) of Cenchrus ciliaris of the Texas cultivar from which caryopses were extracted to perform th...
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| Format: | Online |
| Language: | spa |
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Facultad de Ciencias Agropecuarias
2019
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| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/24833 |
| _version_ | 1811172634849705984 |
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| author | Agüero, César Germán |
| author_facet | Agüero, César Germán |
| author_sort | Agüero, César Germán |
| collection | Portal de Revistas |
| description | The objective of the study was to establish guidelines for the viability test of seeds by tetrazolium in Cenchrus ciliaris using the Panicum protocols (ISTA, 2012). We worked with fascicles (dispersion unit) of Cenchrus ciliaris of the Texas cultivar from which caryopses were extracted to perform the tests. The appropriate method for cutting caryopses and the staining times were determined and two concentrations of tetrazolium solution were tested. In addition, after adjusting the conditions, evaluation protocols were developed. The adequate conditions to perform the viability test are 18 h of seed wetting between papers at a temperature of 20 °C, incomplete longitudinal cut through the embryo, tetrazolium concentration at 0.5 or 1 % and 10 hours of staining at 30 °C. The staining maps allow differentiating accurately the viability of the caryopses. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-24833 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2019 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-248332020-03-30T18:07:59Z Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds Ajuste de las especificaciones técnicas del test de viabilidad por tetrazolio en semillas de Cenchrus ciliaris L. (buffel grass) Agüero, César Germán Cenchrus ciliaris viability tetrazolium caryopses protocols Cenchrus ciliaris viabilidad tetrazolio cariópsides protocolos The objective of the study was to establish guidelines for the viability test of seeds by tetrazolium in Cenchrus ciliaris using the Panicum protocols (ISTA, 2012). We worked with fascicles (dispersion unit) of Cenchrus ciliaris of the Texas cultivar from which caryopses were extracted to perform the tests. The appropriate method for cutting caryopses and the staining times were determined and two concentrations of tetrazolium solution were tested. In addition, after adjusting the conditions, evaluation protocols were developed. The adequate conditions to perform the viability test are 18 h of seed wetting between papers at a temperature of 20 °C, incomplete longitudinal cut through the embryo, tetrazolium concentration at 0.5 or 1 % and 10 hours of staining at 30 °C. The staining maps allow differentiating accurately the viability of the caryopses. El objetivo del estudio fue establecer lineamientos para realizar el test de viabilidad de semillas por tetrazolio en Cenchrus ciliaris utilizando los protocolos de Panicum (ISTA, 2012). Se trabajó con fascículos (unidad de dispersión) de Cenchrus ciliaris del cultivar Texas de los cuales se extrajeron cariópsides para realizar los ensayos. Se determinó el método apropiado de corte de cariópsides y los tiempos de tinción; se probaron dos concentraciones de solución de tetrazolio. Además, se desarrollaron protocolos de evaluación. Las condiciones adecuadas para realizar el test de viabilidad son: 18 h de humedecimiento entre papel a temperatura de 20 °C; corte longitudinal incompleto a través del embrión; concentración de tetrazolio al 0,5 o 1 % y 10 h de tinción a 30 °C. Los mapas de tinción permiten diferenciar de forma precisa la viabilidad de las cariópsides. Facultad de Ciencias Agropecuarias 2019-12-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/agris/article/view/24833 10.31047/1668.298x.v36.n2.24833 AgriScientia; Vol. 36 No. 2 (2019); 81-88 AgriScientia; Vol. 36 Núm. 2 (2019); 81-88 1668-298X 10.31047/1668.298x.v36.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/24833/29201 https://revistas.unc.edu.ar/index.php/agris/article/view/24833/29281 |
| spellingShingle | Cenchrus ciliaris viability tetrazolium caryopses protocols Cenchrus ciliaris viabilidad tetrazolio cariópsides protocolos Agüero, César Germán Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title | Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title_alt | Ajuste de las especificaciones técnicas del test de viabilidad por tetrazolio en semillas de Cenchrus ciliaris L. (buffel grass) |
| title_full | Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title_fullStr | Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title_full_unstemmed | Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title_short | Adjustment of the technical specifications for the Tetrazolium viability test in Cenchrus ciliaris L. (buffel grass) seeds |
| title_sort | adjustment of the technical specifications for the tetrazolium viability test in cenchrus ciliaris l buffel grass seeds |
| topic | Cenchrus ciliaris viability tetrazolium caryopses protocols Cenchrus ciliaris viabilidad tetrazolio cariópsides protocolos |
| topic_facet | Cenchrus ciliaris viability tetrazolium caryopses protocols Cenchrus ciliaris viabilidad tetrazolio cariópsides protocolos |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/24833 |
| work_keys_str_mv | AT aguerocesargerman adjustmentofthetechnicalspecificationsforthetetrazoliumviabilitytestincenchrusciliarislbuffelgrassseeds AT aguerocesargerman ajustedelasespecificacionestecnicasdeltestdeviabilidadportetrazolioensemillasdecenchrusciliarislbuffelgrass |