In vitro establishment of four olive varieties grown in the field (Olea europea L.)
Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the expla...
| Main Authors: | , |
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| Format: | Online |
| Language: | spa |
| Published: |
Facultad de Ciencias Agropecuarias
2017
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| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/19041 |
| _version_ | 1811172553625960448 |
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| author | Turina, C. A. Bima, P. |
| author_facet | Turina, C. A. Bima, P. |
| author_sort | Turina, C. A. |
| collection | Portal de Revistas |
| description | Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the explants response to two cytokinins (BA, zeatin), two carbon sources (sucrose, mannitol), and activated charcoal (0 y 1 g.L-1), and the impact of the season in which materials were collected. The use of ultrasound during disinfection allowed satisfactory establishment rates (44 %). BA improved sprouting (36 % vs. 24 %), and so did mannitol (75 % vs. 20 %). The addition of activated charcoal did not show statistically significant effects on both survival and sprouting. We observed variations depending on the season of collection and the plant variety. Spring was –for all varieties– the most favourable time to collect explants: the highest sprouting rates (21 % to 62 %) and the lowest contamination levels were obtained, Frantoio and Manzanilla being the varieties least affected by contamination. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-19041 |
| institution | Universidad Nacional de Cordoba |
| language | spa |
| publishDate | 2017 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-190412020-03-31T16:08:43Z In vitro establishment of four olive varieties grown in the field (Olea europea L.) Establecimiento in vitro de cuatro variedades de olivo cultivadas a campo (Olea europea L.) Turina, C. A. Bima, P. ultrasonido zeatina 6-benciladenina manitol carbón activado ultrasound zeatin 6-benzyladenine mannitol activated charcoal. Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the explants response to two cytokinins (BA, zeatin), two carbon sources (sucrose, mannitol), and activated charcoal (0 y 1 g.L-1), and the impact of the season in which materials were collected. The use of ultrasound during disinfection allowed satisfactory establishment rates (44 %). BA improved sprouting (36 % vs. 24 %), and so did mannitol (75 % vs. 20 %). The addition of activated charcoal did not show statistically significant effects on both survival and sprouting. We observed variations depending on the season of collection and the plant variety. Spring was –for all varieties– the most favourable time to collect explants: the highest sprouting rates (21 % to 62 %) and the lowest contamination levels were obtained, Frantoio and Manzanilla being the varieties least affected by contamination. Las plantaciones de olivo (Olea europea L.) presentan problemas sanitarios que comprometen la producción y pueden mitigarse con el saneamiento vegetal mediante la obtención de plantas cultivadas in vitro. El objetivo de este trabajo fue desarrollar un protocolo de establecimiento in vitro de material vegetal proveniente de plantas adultas cultivadas a campo. Se evaluaron cuatro métodos de desinfección y la respuesta de los explantos a las citocininas 6-benciladenina (BA) y zeatina, fuentes de carbono (sacarosa y manitol), al carbón activado (0 y 1 g.L-1) y el efecto de las épocas de recolección de material vegetal. El uso de ultrasonido, durante la desinfección, permitió obtener valores de establecimiento adecuados (44 %). El BA mejoró la brotación (36% vs. 24%), al igual que el manitol (75 % vs. 20 %). El agregado de carbón activado no influenció en la sobrevivencia y brotación. Se observaron diferencias entre las épocas de recolección, influenciadas, además, por la variedad. La primavera fue, para todas las variedades, la época más favorable para la recolección de estacas; se obtuvieron los mayores porcentajes de brotación, desde un 21 a un 62 %, y los menores porcentajes de contaminación, siendo Frantoio y Manzanilla las variedades menos afectadas. Facultad de Ciencias Agropecuarias 2017-12-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/agris/article/view/19041 10.31047/1668.298x.v34.n2.19041 AgriScientia; Vol. 34 No. 2 (2017); 59-68 AgriScientia; Vol. 34 Núm. 2 (2017); 59-68 1668-298X 10.31047/1668.298x.v34.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/19041/18956 https://revistas.unc.edu.ar/index.php/agris/article/view/19041/29319 |
| spellingShingle | ultrasonido zeatina 6-benciladenina manitol carbón activado ultrasound zeatin 6-benzyladenine mannitol activated charcoal. Turina, C. A. Bima, P. In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title | In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title_alt | Establecimiento in vitro de cuatro variedades de olivo cultivadas a campo (Olea europea L.) |
| title_full | In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title_fullStr | In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title_full_unstemmed | In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title_short | In vitro establishment of four olive varieties grown in the field (Olea europea L.) |
| title_sort | in vitro establishment of four olive varieties grown in the field olea europea l |
| topic | ultrasonido zeatina 6-benciladenina manitol carbón activado ultrasound zeatin 6-benzyladenine mannitol activated charcoal. |
| topic_facet | ultrasonido zeatina 6-benciladenina manitol carbón activado ultrasound zeatin 6-benzyladenine mannitol activated charcoal. |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/19041 |
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