Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants
In Argentina, wheat fields have exhibited virus-like symptoms, such as chlorotic streaking, dwarfing, yellowing and empty ears since 2007. Symptomatic plants and leaves samples were collected in Marcos Juárez (2007) and Río Cuarto (2008 and 2013), both of them in Córdoba province. The virus was expe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Online |
| Language: | eng |
| Published: |
Facultad de Ciencias Agropecuarias
2015
|
| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/16561 |
| _version_ | 1811172546855305216 |
|---|---|
| author | Dumón, A.D. Mattio, M.F. Argüello Caro, E.B. Alemandri, V. Puyané, N. del Vas, M. López Lambertini, P. Truol, G. |
| author_facet | Dumón, A.D. Mattio, M.F. Argüello Caro, E.B. Alemandri, V. Puyané, N. del Vas, M. López Lambertini, P. Truol, G. |
| author_sort | Dumón, A.D. |
| collection | Portal de Revistas |
| description | In Argentina, wheat fields have exhibited virus-like symptoms, such as chlorotic streaking, dwarfing, yellowing and empty ears since 2007. Symptomatic plants and leaves samples were collected in Marcos Juárez (2007) and Río Cuarto (2008 and 2013), both of them in Córdoba province. The virus was experimentally transmitted from symptomatic wheat plants to wheat cv. Baguette 10 and cv. BioINTA 3005 using the vector Delphacodes kuscheli Fennah (Delphacidae); symptoms of chlorotic streaking, dwarfing and yellowing appeared in the inoculated cereals at 10–15 days post-inoculation. Virus presence was confirmed by electron microscopy and RT-PCR using degenerated primers, which amplified a conserved region of the plant rhabdovirus polymerase (L) gene. Sequence comparison showed 98% nucleotide identity with Maize yellow striate virus C. Caroya (JQ715419) isolated from corn in Argentina. To our knowledge, this is the first report of the occurrence of Maize yellow striate virus in wheat in Argentina. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-16561 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2015 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-165612020-02-13T20:56:55Z Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants Aparición de un aislado estrechamente relacionado con el Maize yellow striate virus en plantas de trigo Dumón, A.D. Mattio, M.F. Argüello Caro, E.B. Alemandri, V. Puyané, N. del Vas, M. López Lambertini, P. Truol, G. Triticum aestivum Cytorhabdovirus gen de la polimerasa Delphacodes kuscheli. Triticum aestivum Cytorhabdovirus polymerase gene Delphacodes kuscheli. In Argentina, wheat fields have exhibited virus-like symptoms, such as chlorotic streaking, dwarfing, yellowing and empty ears since 2007. Symptomatic plants and leaves samples were collected in Marcos Juárez (2007) and Río Cuarto (2008 and 2013), both of them in Córdoba province. The virus was experimentally transmitted from symptomatic wheat plants to wheat cv. Baguette 10 and cv. BioINTA 3005 using the vector Delphacodes kuscheli Fennah (Delphacidae); symptoms of chlorotic streaking, dwarfing and yellowing appeared in the inoculated cereals at 10–15 days post-inoculation. Virus presence was confirmed by electron microscopy and RT-PCR using degenerated primers, which amplified a conserved region of the plant rhabdovirus polymerase (L) gene. Sequence comparison showed 98% nucleotide identity with Maize yellow striate virus C. Caroya (JQ715419) isolated from corn in Argentina. To our knowledge, this is the first report of the occurrence of Maize yellow striate virus in wheat in Argentina. En la Argentina, desde 2007 se han observado síntomas similares a virus en campos de trigo, tales como rayas cloróticas, enanismo y amarillamiento. Se tomaron muestras de plantas y hojas sintomáticas de Marcos Juárez (2007) y de Río Cuarto (2008 y 2013), ambas localidades de la provincia de Córdoba. El virus fue transmitido experimentalmente a partir de plantas de trigo sintomáticas a los cultivares Baguette 10 y BIOINTA 3005, utilizando el vector Delphacodes kuscheli Fennah (Delphacidae); los síntomas de rayas cloróticas, enanismo y amarillamiento en los cereales inoculados se reprodujeron a los 10-15 días posinoculación. La presencia de virus se confirmó por microscopía electrónica y RT-PCR usando cebadores degenerados, que amplifican una región conservada del gen de la polimerasa (L) de los rhabdovirus de plantas. La comparación de secuencias mostró 98% de identidad de nucleótidos con el Maize yellow striate virus C. Caroya (JQ715419) aislado de maíz en Argentina. Hasta el momento, este es el primer reporte de la presencia del Maize yellow striate virus en trigo en la Argentina. Facultad de Ciencias Agropecuarias 2015-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/16561 10.31047/1668.298x.v32.n2.16561 AgriScientia; Vol. 32 No. 2 (2015); 107-112 AgriScientia; Vol. 32 Núm. 2 (2015); 107-112 1668-298X 10.31047/1668.298x.v32.n2 eng https://revistas.unc.edu.ar/index.php/agris/article/view/16561/16371 |
| spellingShingle | Triticum aestivum Cytorhabdovirus gen de la polimerasa Delphacodes kuscheli. Triticum aestivum Cytorhabdovirus polymerase gene Delphacodes kuscheli. Dumón, A.D. Mattio, M.F. Argüello Caro, E.B. Alemandri, V. Puyané, N. del Vas, M. López Lambertini, P. Truol, G. Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title | Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title_alt | Aparición de un aislado estrechamente relacionado con el Maize yellow striate virus en plantas de trigo |
| title_full | Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title_fullStr | Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title_full_unstemmed | Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title_short | Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants |
| title_sort | occurrence of a closely related isolate to maize yellow striate virus in wheat plants |
| topic | Triticum aestivum Cytorhabdovirus gen de la polimerasa Delphacodes kuscheli. Triticum aestivum Cytorhabdovirus polymerase gene Delphacodes kuscheli. |
| topic_facet | Triticum aestivum Cytorhabdovirus gen de la polimerasa Delphacodes kuscheli. Triticum aestivum Cytorhabdovirus polymerase gene Delphacodes kuscheli. |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/16561 |
| work_keys_str_mv | AT dumonad occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT mattiomf occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT arguellocaroeb occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT alemandriv occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT puyanen occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT delvasm occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT lopezlambertinip occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT truolg occurrenceofacloselyrelatedisolatetomaizeyellowstriatevirusinwheatplants AT dumonad apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT mattiomf apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT arguellocaroeb apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT alemandriv apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT puyanen apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT delvasm apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT lopezlambertinip apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo AT truolg apariciondeunaisladoestrechamenterelacionadoconelmaizeyellowstriatevirusenplantasdetrigo |