Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina
Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified...
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | eng |
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Facultad de Ciencias Agropecuarias
2015
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| Subjects: | |
| Online Access: | https://revistas.unc.edu.ar/index.php/agris/article/view/16558 |
| _version_ | 1811172546075164672 |
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| author | de Breuil, S. La Rossa, F.R. Giudici, A. Wulff, A. Bejerman, N. Giolitti, F. Lenardon, S. |
| author_facet | de Breuil, S. La Rossa, F.R. Giudici, A. Wulff, A. Bejerman, N. Giolitti, F. Lenardon, S. |
| author_sort | de Breuil, S. |
| collection | Portal de Revistas |
| description | Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified by serological and molecular tests. Nucleotide and derived amino acid sequence analyses of the nucleocapsid (N) gene indicated a high degree of identity between the GRSV peanut isolates, indicating that there is no molecular variability in the N gene of the GRSV that infects peanuts in the cropping area of Córdoba. In this study, we determined the presence of thrips species in the crop, which can potentially transmit the virus. Thrips were observed in all the evaluated peanut fields. Frankliniella schultzei was the most frequently identified species followed by Caliothrips phaseoli and Frankliniella occidentalis. This work reports the presence of F. schultzei and F. occidentalis in peanuts in Argentina for the first time. These results along with the high degree of similarity between the GRSV peanut isolates suggest that the virus could be transmitted by F. schultzei, which has been cited as its most efficient vector. |
| format | Online |
| id | oai:ojs.revistas.unc.edu.ar:article-16558 |
| institution | Universidad Nacional de Cordoba |
| language | eng |
| publishDate | 2015 |
| publisher | Facultad de Ciencias Agropecuarias |
| record_format | ojs |
| spelling | oai:ojs.revistas.unc.edu.ar:article-165582020-02-13T21:37:39Z Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina Análisis filogenético de aislamientos de Groundnut ringspot virus desde maní e identificación de posibles trips vectores asociados al cultivo de maní en la Argentina de Breuil, S. La Rossa, F.R. Giudici, A. Wulff, A. Bejerman, N. Giolitti, F. Lenardon, S. Arachis hypogaea Tospovirus caracterización molecular Frankliniella schultzei. Arachis hypogaea Tospovirus molecular characterization Frankliniella schultzei. Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified by serological and molecular tests. Nucleotide and derived amino acid sequence analyses of the nucleocapsid (N) gene indicated a high degree of identity between the GRSV peanut isolates, indicating that there is no molecular variability in the N gene of the GRSV that infects peanuts in the cropping area of Córdoba. In this study, we determined the presence of thrips species in the crop, which can potentially transmit the virus. Thrips were observed in all the evaluated peanut fields. Frankliniella schultzei was the most frequently identified species followed by Caliothrips phaseoli and Frankliniella occidentalis. This work reports the presence of F. schultzei and F. occidentalis in peanuts in Argentina for the first time. These results along with the high degree of similarity between the GRSV peanut isolates suggest that the virus could be transmitted by F. schultzei, which has been cited as its most efficient vector. Groundnut ringspot virus (GRSV, género Tospovirus) es un virus que infecta naturalmente el cultivo de maní (Arachis hypogaea L.) en la región productora de Córdoba, Argentina. En distintas localidades de la provincia, se colectaron 14 aislamientos virales provenientes de maníes que manifestaban síntomas característicos de Tospovirus. Todos los aislamientos virales fueron identificados como GRSV mediante pruebas serológicas y moleculares. El análisis de las secuencias nucleotídicas y de amino ácidos deducidas del gen de la nucleoproteína (N) reveló un alto grado de identidad entre los 14 aislamientos, indicando que no existe variabilidad molecular en el gen N del GRSV que infecta maní en la provincia de Córdoba. En este estudio se determinó la presencia de trips en el cultivo que pueden potencialmente transmitir la enfermedad. Estos insectos fueron observados colonizando maní en todos los lotes evaluados. La especie identificada con mayor frecuencia fue Frankliniella schultzei, seguida de Caliothrips phaseoli y Frankliniella occidentalis. Este es el primer reporte de F. schultzei y F. occidentalis afectando maní en Argentina. Estos resultados, junto con el elevado grado de similitud encontrado entre los distintos aislamientos de GRSV, sugieren que el virus puede ser transmitido por F. schultzei, citado como el vector más eficiente del GRSV. Facultad de Ciencias Agropecuarias 2015-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/16558 10.31047/1668.298x.v32.n1.16558 AgriScientia; Vol. 32 No. 1 (2015); 77-82 AgriScientia; Vol. 32 Núm. 1 (2015); 77-82 1668-298X 10.31047/1668.298x.v32.n1 eng https://revistas.unc.edu.ar/index.php/agris/article/view/16558/16367 |
| spellingShingle | Arachis hypogaea Tospovirus caracterización molecular Frankliniella schultzei. Arachis hypogaea Tospovirus molecular characterization Frankliniella schultzei. de Breuil, S. La Rossa, F.R. Giudici, A. Wulff, A. Bejerman, N. Giolitti, F. Lenardon, S. Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title | Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title_alt | Análisis filogenético de aislamientos de Groundnut ringspot virus desde maní e identificación de posibles trips vectores asociados al cultivo de maní en la Argentina |
| title_full | Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title_fullStr | Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title_full_unstemmed | Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title_short | Phylogenetic analysis of Groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in Argentina |
| title_sort | phylogenetic analysis of groundnut ringspot virus isolates from peanut and identification of potential thrips vectors in peanut crop in argentina |
| topic | Arachis hypogaea Tospovirus caracterización molecular Frankliniella schultzei. Arachis hypogaea Tospovirus molecular characterization Frankliniella schultzei. |
| topic_facet | Arachis hypogaea Tospovirus caracterización molecular Frankliniella schultzei. Arachis hypogaea Tospovirus molecular characterization Frankliniella schultzei. |
| url | https://revistas.unc.edu.ar/index.php/agris/article/view/16558 |
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